Abstract
Evidence is provided which establishes the correctness of the finding that, when malate was synthesized by darkened acidifying CAM leaves in the presence of 14CO2, the distribution of label between carbon atoms 4 and 1 of the malate approximated to a ratio of 2 : 1. This result was not an artefact of the degradation procedure as suggested by Sutton & Osmond (dy1972). As the explanation, that this distribution of label resulted from a double carboxylation process, has not found support from recent work in other laboratories, it would seem advisable to seek an alternative hypothesis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Avadhani, P.N. (1957): Studies on the fixation of carbon dioxide in leaves and roots of Kalanchoë and roots of Ricinus. Ph. D. thesis. University of Durham.
Bradbeer, G. (1957): Some interrelations of fat, carbohydrate and organic acid metabolism in etiolated Helianthus annum seedlings. Ph.D. thesis. University of Durham.
Bradbeer, J.W. (1954): Some carbohydrate and acid conversions in Kalanchoë crenata. Ph.D. thesis. University of Durham
Bradbeer, J.W. (1963): Physiological studies on acid metabolism in green plants. IX. The distribution of 14C in malate of darkened Kalanchoë leaf fragments after infiltration with labelled pyruvate. Proc. Roy. Soc. B., 157: 279–289.
Bradbeer, J.W., S.L. Ranson & Mary Stiller (1958): Malate synthesis in Crassulacean leaves. I. The distribution of C14 in malate of leaves exposed to C14O2 in the dark. Plant Physiol., 33: 66–70.
Cockburn, W. (1965): Problems related to acid synthesis in higher green plants. Ph.D. thesis. University of Newcastle.
Jolchine, G. (1959): Sur la distribution du 14C dans les molécules d’acide malique synthétisées par fixation de 14CO2 dans les feuilles de Bryophyllum daigremontianum Berger. Bull. Soc. Chim. biol., 41: 227–234.
Nossal, P.M. (1952): Estimation of L-malate and fumarate by malic decarboxylase of Lactobacillus arabinosus. Biochem. J., 50: 349–355.
Quayle, J.R., R.C. Fuller, A.A. Benson & M. Calvin (1954): Enzymatic carboxylation of ribulose diphosphate. J. Am. Chenu Soc, 76: 3610–3611.
Stiller, M. (1956): Experiments on the relationship between succulence and acid accumulation in Bryophyllum with an addendum on the mechanism of acid accumulation. M.S. thesis. Purdue University.
Sutton, B.G. & C.B. Osmond (1972): Dark fixation of CO2 by Crassulacean plants. Evidence for a single carboxylation step. Plant Physiol., 50: 360–365.
Walker, D.A. (1957): Physiological studies on acid metabolism. 4. Phosphoenolpyruvic carboxylase activity in extracts of Crassulacean plants. Biochem. J., 67: 73–79.
Weissbach, A., P.Z. Smyrniotis & B.L. Horrecker (1954): Pentose phosphate and CO2 fixation with spinach extracts. J. Am. Chem. Soc., 76: 3611–3612.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1975 Dr. W. Junk b.v., Publishers, The Hague
About this paper
Cite this paper
Bradbeer, J.W., Cockburn, W., Ranson, S.L. (1975). The Labelling of the Carboxyl Carbon Atoms of Malate in Kalanchoë crenata Leaves. In: Marcelle, R. (eds) Environmental and Biological Control of Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1957-6_26
Download citation
DOI: https://doi.org/10.1007/978-94-010-1957-6_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-6193-179-9
Online ISBN: 978-94-010-1957-6
eBook Packages: Springer Book Archive