Abstract
Plastic microfluidic devices with 18-cm separation length and an offset “double-T” injection system were used to achieve DNA sequencing in 30 minutes with 98%-correct identification to a read length of 650 bases. We recently demonstrated two proteomics-enabling technologies on plastic fluidic devices: isoelectric focusing (IEF) and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Manz, A.; Fettinger, J. C.; et al. Trends in Anal. Chem. 1991, 10, 144–149.
Harrison, D. J.; Manz, A.; Fan, Z.; et al. Anal. Chem. 1992, 64, 1926–1932.
Woolley, A. T.; Mathies, R. A. Proc. Natl. Acad. Sci. USA 1994, 91, 11348–11352.
Backhouse, C; Caamano, M.; Oaks, F.; et al. Electrophoresis 2000, 21, 150–156.
Liu, S.; Ren, H.; Gao, Q.; et al. Proc. Natl. Acad. Sci. USA 2000, 97, 5369–5374.
Kroutchinina, N.; Jacobson, S.C.; et al. Anal. Chem. 2001, 73, 2669–2674.
Bousse, L.; Mouradian, S.; Minalla, A.; et al. Anal. Chem. 2001, 73; 1207–1212.
Burns, M.; Johnson, B.; Brahmasandra, S.; et al. Science 1998, 282, 484–487.
McCormick, R.; Nelson, R.; Alonso, M. G.; et al. Anal. Chem. 1997, 69, 2626.
McDonald, J. C.; Duffy, D. C.; et al. Electrophoresis 2000, 21, 27–40.
Soper, S. A.; Ford, S. M.; Qi, S.; et al. Anal Chem. 2000, 72, 643 A–651 A.
Sassi, A.; Cruzado, I.; Björnson, T.; et al. J. Chromatogr. A, 2000, 894, 203–217.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this paper
Cite this paper
Fan, Z.H. et al. (2001). Plastic Microfluidic Devices for DNA Sequencing and Protein Separations. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_7
Download citation
DOI: https://doi.org/10.1007/978-94-010-1015-3_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3893-5
Online ISBN: 978-94-010-1015-3
eBook Packages: Springer Book Archive