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Integrated PCR-CE System for DNA Analysis to the Single Molecule Limit

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Book cover Micro Total Analysis Systems 2001

Abstract

We have developed an integrated PCR-CE system for DNA amplification and analysis from nanoliter volumes with single molecule sensitivity. In addition, thermal cycling elements have been microfabricated directly onto the device for improved thermal cycling efficiency. PCR-CE data demonstrating sex determination from human genomic DNA are presented.

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References

  1. A. T. Woolley, D. Hadley, P. Landre, A. J. deMello, R. A. Mathies and M. A. Northrup, Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device, Anal. Chem., 68, pp. 4081–4086, (1996).

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  4. D. Neeser and S. Liechtigallati, Sex Determination of Forensic Samples by Simultaneous PCR Amplification of a-Satellite DNA from Both the X and Y Chromosomes, J. Forensic Sci., 40, pp. 239–241, (1995).

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© 2001 Springer Science+Business Media New York

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Lagally, E.T., Mathies, R.A. (2001). Integrated PCR-CE System for DNA Analysis to the Single Molecule Limit. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_46

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  • DOI: https://doi.org/10.1007/978-94-010-1015-3_46

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3893-5

  • Online ISBN: 978-94-010-1015-3

  • eBook Packages: Springer Book Archive

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