Abstract
As part of a larger effort to perform isoelectric focusing and electrophoresis in a microfluidic device [1,2], image analysis techniques that permit non-invasive quantitative measurement of pH in a micro fluidic channel have been developed [3]. By using two independent color channels, the ratio of the two forms of the indicator dye can be measured; this ratio is then correlated to pH. Methods for accounting for illumination variation have also been developed and shown to reduce scatter in the data. Results of this technique are compared to model predictions of dye concentration and pH gradients and found to be in good agreement.
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References
C.R. Cabrera, P. Yager, Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques, Electrophoresis, 22, pp. 355–362, (2001).
K. Macounova, C.R. Cabrera, P. Yager, Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF, Analytical. Chemistry, 73, pp. 1627–1633, (2001).
C.R. Cabrera, B. Finlayson, P. Yager, Formation of natural pH gradients in a microfluidic device under flow conditions. Model and experimental validation, Analytical Chemistry, 73, pp. 658–666, (2001).
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© 2001 Springer Science+Business Media New York
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Cabrera, C.R., Yager, P. (2001). Mapping of pH Gradients in Microfluidic Electrokinetic Devices. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_40
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DOI: https://doi.org/10.1007/978-94-010-1015-3_40
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3893-5
Online ISBN: 978-94-010-1015-3
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