Abstract
A technique for protein identification through enzymatic digestion in nanovial (nL) arrays and analysis with MALDI-TOF MS is presented. Through micro-dispensing of low-level protein solutions (10 nM—500 nM) and simultaneous evaporation the protein is concentrated up to 300 times in-vial, which increases Michaelis-Menten kinetics, resulting in identification after 0.2–10min digestions.
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© 2001 Springer Science+Business Media New York
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Ericsson, D., Ekström, S., Nilsson, J., Bergquist, J., Marko-Varga, G., Laurell, T. (2001). Dispenser-Aided Nanodigestion For Rapid Protein Identification in MALDI-TOF Nanovial Arrays. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_31
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DOI: https://doi.org/10.1007/978-94-010-1015-3_31
Publisher Name: Springer, Dordrecht
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