Abstract
Cell-free protein synthesis is one of the most useful techniques to amplify proteins and to characterize the mechanisms of gene expression. The synethesis reactions are performed in parallel microreactors fabricated on a PDMS-glass hybrid microchip. To achieve the favorable reaction condition heat control devices are integrated on the glass substrate of the hybrid structure. It is demonstrated that fluorescent proteins, GFP and BFP, can be successfully synthesized in the present microreactors.
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© 2001 Springer Science+Business Media New York
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Yamamoto, T., Nojima, T., Fujii, T. (2001). Cell-Free Protein Synthesis in PDMS-Based Parallel Microreactors. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_24
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DOI: https://doi.org/10.1007/978-94-010-1015-3_24
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3893-5
Online ISBN: 978-94-010-1015-3
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