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Field-Deployable Amperometric Enzyme Electrodes for Direct Monitoring of Organophosphate Nerve Agents

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Enzymes in Action

Part of the book series: NATO Science Partnership Sub-Series: 1: ((ASDT,volume 33))

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Abstract

Amperometric enzyme electrodes for field, on-site and in-situ/on-line remote, monitoring of organophosphate nerve agents is reported. The sensors rely upon the coupling of the rapid biocatalytic hydrolysis of the organophosphates by the immobilized organophosphorus hydrolase and the sensitive and rapid anodic detection of p-nitrophenol product. The biosensors are fast responding, highly selective and show sub-micromolar lower detection limit for paraoxon and methyl parathion. The low detection limits coupled with high selectivity and faster and simplified operation are the benefits of the developed biosensors over the analogous enzyme inhibition biosensors make them ideal for field monitoring of organophosphate compounds.

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References

  1. Food and Agricultural Organization of the United Nations, Rome. (1989)FAO Prod. Yearb., 43, 320.

    Google Scholar 

  2. United States Department of Agriculture. (1992). In: Agrcultural Statistics. United States Government Printing Office, Washington, D.C., pp. 395

    Google Scholar 

  3. Compton, J.A. (1988) In: Military Chemical and Biological Agents. Telford Press, NJ, pp. 135.

    Google Scholar 

  4. Diehl-Faxon, J., Ghindilis, A.L., Atanasov, P. and Wilkins, E. (1996) Direct electron transfer based tri-enzyme electrode for monitoring of organophosphorus pesticides. Sens. Actuators B 35-36, 448-457.

    Google Scholar 

  5. Paddle, B.M. (1996) Biosensors for chemical and biological agents of defence interest. Biosens. Bioelectron., 11 1079-1113.

    Article  CAS  Google Scholar 

  6. Mulchandani, P., Mulchandani, A., Kaneva, I. and Chen, W. (1998) Biosensor for direct determination of organophosphate nerve agents. 1. Potentiometric enzyme electrode. Biosens. Bioelectron.14, 77-85.

    Article  Google Scholar 

  7. Mulchandani, A., Mulchandani, P. Kaneva, I. and W. Chen. (1998) Biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 1. Potentiometric microbial electrode. Anal. Chem 70, 4140-4145.

    Article  CAS  Google Scholar 

  8. Mulchandani, A., Mulchandani, P., Chauhan, S., Kaneva, I. and Chen, W. (1998) A potentiometric microbial biosensor for direct determination of organophosphate nerve agents. Electroanalysis 10, 733-737.

    Article  CAS  Google Scholar 

  9. Mulchandani, A., Kaneva, I. and Chen, W. (1998) Microbial biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 2. Fiber-optic microbial biosensor. Anal. Chem. 70, 5042-5046.

    Article  CAS  Google Scholar 

  10. Rogers, K., Wang, Y., Mulchandani, A., Mulchandani, P. and Chen, W. (1999) Development of a versatile organophosphorus hydrolase-based assay for organophosphate pesticides. Biotechnol. Prog. 15 517-522.

    Article  CAS  Google Scholar 

  11. Mulchandani, A., Pan, S. and Chen, W. (1999) Fiber-optic biosensor for direct determination of organophosphate nerve agents. Biotechnol. Prog. 15, 130-134.

    Article  CAS  Google Scholar 

  12. Dave, K.I, Miller, C.E. and Wild, J.R. (1993) Characterization of organophosphorus hydrolases and the genetic manipulation of the phosphotriesterase from Pseudomonas diminuta. Chem.-Biol. Interactions. 87, 55-68.

    CAS  Google Scholar 

  13. Wang, J. and Chen, Q. (1995) Remote electrochemical biosensor for field monitoring of phenolic compounds. Anal. Chimica Acta 312 39-44

    Article  CAS  Google Scholar 

  14. Wang, J., Cerpia, G. and Chen, Q. (1996) Submersible bioprobe for continuous monitoring of peroxide species. Electroanalysis 8 124-127

    Article  CAS  Google Scholar 

  15. Wang, J. (1997) Remote electrochemical sensors for monitoring inorganic and organic pollutants. Trends Anal. Chem. 16 84-88.

    Article  CAS  Google Scholar 

  16. Wang, J., Tian, B., Lu, J.MacDonald, D., Wang, J. and Luo, D.(1998) Renewable-reagent enzyme inhibition sensor for remote monitoring of cyanide.Electroanalysis 10 1034-1037

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Environmental Engineering University of California, Riverside, CA, 92521, USA

    Ashok Mulchandani, Priti Mulchandani & Wilfred Chen

  2. Department of Chemical and Biochemistry, New Mexico State University, Las Cruces, NM, 880003, USA

    Joseph Wang & Liang Chen

Authors
  1. Ashok Mulchandani
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  2. Priti Mulchandani
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  3. Wilfred Chen
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  4. Joseph Wang
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  5. Liang Chen
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Editor information

Editors and Affiliations

  1. Department of Organic Chemistry, NSR Institute for Molecular Structure, Design and Synthesis, University of Nijmegen, The Netherlands

    Binne Zwanenburg

  2. Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Łódź, Poland

    Marian Mikołajczyk  & Piotr Kiełbasiński  & 

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© 2000 Springer Science+Business Media Dordrecht

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Mulchandani, A., Mulchandani, P., Chen, W., Wang, J., Chen, L. (2000). Field-Deployable Amperometric Enzyme Electrodes for Direct Monitoring of Organophosphate Nerve Agents. In: Zwanenburg, B., Mikołajczyk, M., Kiełbasiński, P. (eds) Enzymes in Action. NATO Science Partnership Sub-Series: 1:, vol 33. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0924-9_14

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  • DOI: https://doi.org/10.1007/978-94-010-0924-9_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-6696-6

  • Online ISBN: 978-94-010-0924-9

  • eBook Packages: Springer Book Archive

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