Skip to main content

The Potential of Different Biotechnology Methods in BTW Agent Detection

DNA Methods: Gene Probes

  • Chapter
The Role of Biotechnology in Countering BTW Agents

Part of the book series: NATO Science Series ((ASDT,volume 34))

  • 122 Accesses

Abstract

The 1991 Gulf War and recent disclosures about the BW programs of Iraq and the former Soviet Union have highlighted the need for an effective biological warfare (BW) defense capability. Ideally this should include battlefield detectors as part of an integrated system with physical protection and medical countermeasures, together with a strengthened Biological and Toxin Weapons Convention (BTWC) to reduce the risk of BW proliferation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Miller, L. (1998) Sampling and analysis under a compliance protocol for the Biological and Toxin Weapons Convention, in Proceedings of the 20th ESARDA Annual Meeting - Seminar onModern Verification Techniques: Similarities Synergies and Challenges(forthcoming).

    Google Scholar 

  2. Perry, R.D. and Fetherston, J.D. (1997) Yersinia pestis — Etiologic agent of plagueClinical Microbiology Reviews10, 35–66.

    Google Scholar 

  3. Southern, E.M. (1974) Detection of species specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology98, 505–517.

    Google Scholar 

  4. Tenover, F.C. (1988) Diagnostic deoxyribonucleic acid probes for infectious diseasesClinical Microbiology Reviews1, 82–101.

    Google Scholar 

  5. Graham, C.R., Leslie, D., and Squirrell, D.J. (1992) Gene probe assays on a fibre-optic evanescent wave biosensorBiosensors & Bioelectronics7, 487–493.

    Article  Google Scholar 

  6. Heller, M.J. (1996) A microelectronic device for DNA hybridisation diagnostics, inBiochip Array TechnologiesInternational Business Communications, Southborough.

    Google Scholar 

  7. Evans, A.G. and Charles, S.A. (1990) The application of a rapid, homogenous biosensor based on surface plasmon resonance to clinical chemistry, DNA probes and immunoassayAbstracts of 1st World Congress on BiosensorsElsevier, New York, 223–226.

    Google Scholar 

  8. .Johannsson, A., Ellis, D.H., Bates, D.L., Plumb, A.M., and Stanley, C.J. (1986) Enzyme amplification for immunoassays,.I.Immunological Methods87, 7-1 1.

    Article  Google Scholar 

  9. Squirrell, D.J. and Murphy, M.J. (1994) Adenylate kinase as a cell marker in bioluminescent assays, in A.K. Campbell, L.J. Kricka and P.E. Stanley (eds.)Bioluminescence and ChemiluminescenceJohn Wiley & Sons, Chichester, pp. 486–489.

    Google Scholar 

  10. Saiki, R.K., Scharf, S., Faloona, F., Mullis, K.B., Horn, G.T., Erlich, H.A., and Arnheim, N. (1985) Enzymatic amplification of 3-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anaemiaScience230, 1350–1354.

    Article  Google Scholar 

  11. B.A. White (ed.) (1993) PCR Protocols. Current methods and applications, inMethods in Microbiology15, Academic Press, Harcourt Brace and Company, Ltd., London.

    Google Scholar 

  12. Fulop, M., Leslie, D., and Titball, R. (1996) A rapid, highly sensitive method for the detection ofFrancisella tularensisin clinical samples using the polymerase chain reactionAmerican J. Tropical Medicine and Hygiene54, 364–366.

    Google Scholar 

  13. Northrup, M.A. (1996)Advantages afforded by DNA instruments miniaturisation, in Biochip Array Technologies, International Business Communications, Southborough.

    Google Scholar 

  14. Fulop, L., Barrett, A.D.T., Phillpotts, R., Martin, K., Leslie, D., and Titball, R.W. (1993) Rapid identification of flaviviruses based on conserved NS5 sequences,.1Virological Methods44, 179–188.

    Article  Google Scholar 

  15. Waterhouse, R.N. and Glover, L.A. (1993) Identification of prokaryotic repetitive DNA suitable for use as fingerprinting probesApplied and Environmental Microbiology59, 1391–1397.

    Google Scholar 

  16. Pääbo, S., Higuchi, R.G., and Wilson, A.C. (1989) Ancient DNA and the polymerase chain reaction.The emerging field of molecular archaeologyI Biological Chemistry264, 9709–9712.

    Google Scholar 

  17. McGregor, D.P., Forster, S., Steven, J., Adair, J., Leary, S.E.C., Leslie, D.L., Harris, W.J., and Titball, R.W. (1996) Simultaneous detection of microorganisms in soil extract based on PCR amplification of bacterial 16S rRNA fragmentsBiotechniques21, 436–466.

    Google Scholar 

  18. Wittwer, C.T., Herrman, M.G., Moss, A.A., and Rasmussen, R.P. (1997) Continuous fluorescence monitoring of rapid cycle DNA amplificationBiotechniques22, 130–131.

    Google Scholar 

  19. Tyagi, S. and Kramer, F.R. (1996) Molecular beacons: probes that fluoresce upon hybridizationNature Biotechnology14, 303–308.

    Article  Google Scholar 

  20. Cardullo, R.A., Agrawal, S., Flores, C., Zamecnik, P.C., and Wolf, D.E. (1988) Detection of nucleic acid hybridization by nonradioactive fluorescence resonance energy transferProceedings of the National Academy of Sciences, USA85, 8790–8794.

    Article  Google Scholar 

  21. Bassam, B.J., Allen, T., Flood, S., Stevens, J., Wyatt, P., and Livak, K.J. (1996) Nucleic acid sequence detection systems: revolutionary automation for monitoring and reporting PCR productsAustralasian Biotechnology6, 285–294.

    Google Scholar 

  22. Wittwer, C.T., Ririe, K.M., Andrew, RV., David, D.A., Gundry, R.A., and Balis, U.J. (1997) The LightcyclerTMa microvolume multisample fluorimeter with rapid temperature controlBiotechniques22, 176–181.

    Google Scholar 

  23. Johns, M., Harrington, L., Titball, R.W., and Leslie, D.L. (1994) Improved methods for the detection ofBacillus anthracisspores by the polymerase chain reactionLetters in Applied Microbiology18, 236–238.

    Article  Google Scholar 

  24. Jackson, P.J., Hugh-Jones, M.E., Adair, D.M., Green, G., Hill, K.K., Kuske, C.R., Grinberg, L.M., Abramova, F.A., and Keim, P. (1998) PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: the presence of multipleBacillus anthracisstrains in different victimsProceedings of the National Academy of Sciences, USA95, 1224–1229.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Leslie, D. (2001). The Potential of Different Biotechnology Methods in BTW Agent Detection. In: Kelle, A., Dando, M.R., Nixdorff, K. (eds) The Role of Biotechnology in Countering BTW Agents. NATO Science Series, vol 34. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0775-7_8

Download citation

  • DOI: https://doi.org/10.1007/978-94-010-0775-7_8

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-6906-6

  • Online ISBN: 978-94-010-0775-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics