Skip to main content
SpringerLink
Log in
Book cover

Micro Total Analysis Systems 2002 pp 614–616Cite as

A 15-Second Protein Separation Employing Hydrodynamic Force on A Microchip

  • Conference paper

Abstract

We report a novel pressurization technique for microchip electrophoresis (μ-CE) that enables the separation of many biological samples (12-96 samples) involving complex mixtures of proteins (>20 proteins) in a single run only taking 15 seconds (s). Although μ-CE relies almost exclusively on electrokinetic forces and some loss of resolution is unavoidable, the method reported here employing hydrodynamic force has enabled a dramatic reduction in separation time without compromising resolution.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  1. Harrison, D. J. et al. Science, 261, 895–897 (1993).

    Article  CAS  Google Scholar 

  2. Ross, D., Johnson, T. J., Locascio, L E. Anal. Chem. 73, 2509–2515 (2001).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan, CREST, Japan Science and Technology Corporation, JST, Japan

    Mari Tabuchi & Yoshinobu Baba

  2. Department of Biochemistry & Biomolecular Recognition, Yamaguchi University School of Medicine, Ube, Japan

    Yasuhiro Kuramitsu & Kazuyuki Nakamura

  3. Central Laboratory for Biomedical Research and Education, Yamaguchi University School of Medicine, Japan

    Kazuyuki Nakamura

Authors
  1. Mari Tabuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuhiro Kuramitsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuyuki Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yoshinobu Baba
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The University of Tokushima, Tokushima, Japan

    Yoshinobu Baba

  2. Waseda University, Tokyo, Japan

    Shuichi Shoji

  3. University of Twente, Enschede, The Netherlands

    Albert van den Berg

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer Science+Business Media Dordrecht

About this paper

Cite this paper

Tabuchi, M., Kuramitsu, Y., Nakamura, K., Baba, Y. (2002). A 15-Second Protein Separation Employing Hydrodynamic Force on A Microchip. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0504-3_4

Download citation

  • DOI: https://doi.org/10.1007/978-94-010-0504-3_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3953-6

  • Online ISBN: 978-94-010-0504-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation