Abstract
The dynamic binding of biotin-4-fluorescein (B4F) (Molecular Probes Europe BV) with 2-um streptavidin-coated (SC) beads (Polysciences) by using a continuous flow method is presented. A recirculating flow is created between micro-etched difruser/nozzle elements. Polystyrene beads (2-μm diameter) are concentrated and held in place in a particular flow pattern while being sequentially perfused by different solutions.
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References
G. Lettieri et al., Proc. Micro Total Analysis Systems 2000, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 351–354 (2000).
G.-L. Lettieri et al., Proc. Micro Total Analysis Systems 2001}, Kluwer Academic Publishers, pp. 503–504, (2001).
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© 2002 Springer Science+Business Media Dordrecht
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Lettieri, GL., de Rooij, N.F., Verpoorte, E. (2002). Microfluidic Device for Bioanalysis Using Freely Moving Beads Trapped in A Recirculating Flow. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0504-3_10
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DOI: https://doi.org/10.1007/978-94-010-0504-3_10
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