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Synthesis of a Covalently Reactive Antigen Analog Derived from a Conserved Sequence of HIV-1 gp120

  • Hiroaki Taguchi
  • Yasuhiro Nishiyama
  • Gary S. Burr
  • Sangeeta A. Karle
  • Sudhir Paul
Part of the American Peptide Symposia book series (APSY, volume 7)

Abstract

Antibodies (Abs) and their light (L) chain subunits are reported to catalyze the cleavage of VIP, the HIV coat proteins gp41 and gpl20, Arg-vasopressin, thyroglobulin, factor VIII, prothrombin and various model peptidase substrates. The serine protease inhibitor diisopropylfluorophosphate (DFP) consistently inhibits the catalytic activity of the Abs, and a serine protease-like catalytic triad in a model proteolytic Ab L chain has previously been deduced from site-directed mutagenesis studies. The catalytic site appears to be germline encoded [1]. In principle, probes that can specifically recognize the active site of serine protease Abs could be applied for the selection of efficient catalytic Abs (CAbs) from display libraries, and perhaps also as the immunogens capable of recruiting the germline encoded Ab variable region genes.

Keywords

Peptide Segment Chain Subunit Ester Phosphonate American Peptide Society Phosphonate Moiety 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Gololobov, G., Sun, M., Paul, S. Mol Immunol 36, 1215–1222 (1999).PubMedCrossRefGoogle Scholar
  2. 2.
    Paul, S., Tramontano, A., Gololobov, G., Zhou, Y., Taguchi, H., Karle, S., Nishiyama, Y., Planque, S., George, S. J. Biol Chem. 276, 28314–28320 (2001).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Hiroaki Taguchi
    • 1
  • Yasuhiro Nishiyama
    • 1
  • Gary S. Burr
    • 1
  • Sangeeta A. Karle
    • 1
  • Sudhir Paul
    • 1
  1. 1.Chemical Immunology Research Center, Department of Pathology and Laboratory MedicineUniversity of Texas-Houston Medical SchoolHoustonUSA

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