Fluorescent Biosensor for CrkII Phosphorylation by the Abl Tyrosine Kinase
Chronic myeloid leukemia (CML) causes the excessive proliferation of myeloid cells ADDIN ENRfu . The majority of CML cases are caused by the reciprocal chromosomal translocation of the first exon of c-Abl on chromosome 9 and the breakpoint cluster region (Bcr) of chromosome 22. The c-Abl gene encodes a nonreceptor kinase that catalyzes the transfer of phosphate from ATP to tyrosine residues on protein substrates. The Bcr-Abl fusion protein produces an overactive kinase, and this deregulated tyrosine kinase activity is required for leukemic transformation.