Advertisement

Fluorescent Biosensor for CrkII Phosphorylation by the Abl Tyrosine Kinase

  • Roseanne M. Hofmann
  • Graham J. Cotton
  • William Bornman
  • Emmanual Chang
  • Tom W. Muir
Part of the American Peptide Symposia book series (APSY, volume 7)

Abstract

Chronic myeloid leukemia (CML) causes the excessive proliferation of myeloid cells ADDIN ENRfu [1]. The majority of CML cases are caused by the reciprocal chromosomal translocation of the first exon of c-Abl on chromosome 9 and the breakpoint cluster region (Bcr) of chromosome 22. The c-Abl gene encodes a nonreceptor kinase that catalyzes the transfer of phosphate from ATP to tyrosine residues on protein substrates. The Bcr-Abl fusion protein produces an overactive kinase, and this deregulated tyrosine kinase activity is required for leukemic transformation.

Keywords

Chronic Myeloid Leukemia Breakpoint Cluster Region High Throughput Screening Assay Fluorescent Biosensor Express Protein Ligation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Mauro, M.J., Druker, B.J. Curr. Oncol Rep. 3:223–227 (2001).PubMedCrossRefGoogle Scholar
  2. 2.
    Buchdunger, E., et al. J. Pharmacol Exp. Ther. 95:139–145 (2000).Google Scholar
  3. 3.
    Druker, B.J., et al. N. Engl J. Med. 344:1031–1037 (2001).PubMedCrossRefGoogle Scholar
  4. 4.
    Druker, B.J., et al. N. Engl J. Med. 344:1038–1042 (2001).PubMedCrossRefGoogle Scholar
  5. 5.
    Knight, Z.A. Blood 96:4003–4005 (2000).PubMedGoogle Scholar
  6. 6.
    le Coutre, P., et al. Blood 95:1758–1766 (2000).PubMedGoogle Scholar
  7. 7.
    Weisberg, E., Griffin, J.D. Blood 95:3498–3505 (2000).PubMedGoogle Scholar
  8. 8.
    Feller, S.M., Knudsen, B., Hanafusa, H. Embo J. 13:2341–2351. (1994).PubMedGoogle Scholar
  9. 9.
    Anafi, M., Rosen, M.K., Gish, G.D., Kay, L.E., Pawson, T. J. Biol. Chem. 71:21365–21374 (1996).Google Scholar
  10. 10.
    Cotton, G.J., Muir, T.W. Chem. Biol. 7:253–261 (2000).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Roseanne M. Hofmann
    • 1
  • Graham J. Cotton
    • 1
  • William Bornman
    • 2
  • Emmanual Chang
    • 1
  • Tom W. Muir
    • 1
  1. 1.Laboratory of Synthetic Protein ChemistryRockefeller University of New YorkUSA
  2. 2.Laboratory of Bioorganic ChemistrySloan-Kettering InstituteNew YorkUSA

Personalised recommendations