Generation of Cellulose Membrane-Bound Peptides with Free C-Termini: A Useful Approach for PDZ Binding Studies

  • Liying Dong
  • Prisca Boisguérin
  • Jens Schneider-Mergener
  • Rudolf Volkmer-Engert
Part of the American Peptide Symposia book series (APSY, volume 7)

Abstract

PDZ [1] domains are recently characterized protein-recognition modules which mostly bind to the C-termini of target proteins. This was reported as well for antibodies like mAB TE34 [2].

Keywords

Cellulose Hydrolysis Cysteine Adduct Thioether 

References

  1. 1.
    Songyang, Z., Fanning, A.S., Fu, C., Xu, J., Marfatia, S.M., Chishti, A.H., Crompton, A., Chan, A.C., Anderson, J.M., Cantley, L.C. Science 275, 73–77 (1997).PubMedCrossRefGoogle Scholar
  2. 2.
    Zilber, B., Scherf, T., Levitt, M., Anglister, J. Biochemistry 30, 10032–10041 (1990).CrossRefGoogle Scholar
  3. 3.
    Frank, R. Tetrahedron 48, 9217–9232 (1992).CrossRefGoogle Scholar
  4. 4.
    Hoffmüller, U., Russwurm, M., Kleinjung, F., Ashurst, J., Oschkinat, H., Volkmer-Engert, R., Koesling, D., Schneider-Mergener, J. Angew. Chem., Int. Ed. 38, 2180–2184 (1999).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Liying Dong
    • 1
  • Prisca Boisguérin
    • 1
  • Jens Schneider-Mergener
    • 1
  • Rudolf Volkmer-Engert
    • 1
  1. 1.Institute of Medical Immunology, University Clinic CharitéHumboldt University of BerlinBerlinGermany

Personalised recommendations