HPLC Analysis, Modeling, and Biological Studies of Antiproliferative Heterocyclic Carboxamides
Reversed phase HPLC and MEKC  provide excellent possibilities to measure the hydrophobic properties of compounds, characterizing them directly from the chromatographic retention time. The aim in this respect is to estimate the hydrophobic character of the molecules on the basis of their retention factors (k′) determined in various separation processes . The expected biological activity of the compounds can be reliably predicted on the basis of the measured hydrophobicity data . Another possibility is the use of highly sophisticated computer programs to calculate hydrophobicity on the basis of the structural moieties building up the molecule being investigated.
KeywordsReverse Phase HPLC Retention Factor Hydrophobic Character Furan Derivative Chromatographic Retention Time