Abstract
Doubly dye labeled peptides that utilize FRET quenching have many applications. A new class of efficient quencher molecules, termed Black Hole Quenchers™, have been introduced into peptides via phosphoramidite derivatives coupled to the hydroxy-lic functions of serine, tyrosine, or hydroxyproline residues. The spectral characteristics for the entire family (BHQ-1, BHQ-2, etc.) are matched to sets of fluorophores, providing high sensitivity and low background detection in a variety of fluorogenic assays.
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© 2001 Springer Science+Business Media Dordrecht
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Walton, T.A. et al. (2001). New Quencher Derivatives for Fluorescence Quenching Peptide Assays. In: Lebl, M., Houghten, R.A. (eds) Peptides: The Wave of the Future. American Peptide Symposia, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0464-0_21
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DOI: https://doi.org/10.1007/978-94-010-0464-0_21
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3905-5
Online ISBN: 978-94-010-0464-0
eBook Packages: Springer Book Archive