A Cassette Ribonuclease: Site Selective Cleavage of RNA by a Ribonuclease S-Bearing RNA-Recognition Segment

  • Shiroh Futaki
  • Michihiro Araki
  • Tatsuto Kiwada
  • Ikuhiko Nakase
  • Yukio Sugiura
Part of the American Peptide Symposia book series (APSY, volume 7)

Abstract

Elucidation of the two-dimensional and three-dimensional structures of RNA has recently been required in relation to their therapeutic potential as targets of RNA-oriented molecules, such as antisense nucleic acids and ribozymes. Structural elucidation of RNAs leads to an understanding of the replication mechanisms of RNA viruses, and also provides new target sites for antiviral drugs. We report here the creation of a novel Rnase, in which a conjugate of an RNA recognition peptide and the S-peptide were encapsulated as a cassette in RNase S (Figure 1A).

Keywords

Hydrolysis Electrophoresis Polyacrylamide Pyrimidine Norleucine 

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Shiroh Futaki
    • 1
  • Michihiro Araki
    • 1
  • Tatsuto Kiwada
    • 1
  • Ikuhiko Nakase
    • 1
  • Yukio Sugiura
    • 1
  1. 1.Institute for Chemical ResearchKyoto UniversityUji, KyotoJapan

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