A Novel High Throughput Analysis of Total Nitrogen Content by a Colorimetric Method

  • Kiyoshi Nokihara
  • Tadashi Yasuhara
Part of the American Peptide Symposia book series (APSY, volume 7)


Determination of total nitrogen amounts is routinely carried out for the quality control of pharmaceutical products especially proteins and food products. For this purpose a method originally developed by Kjeldahl [1] has been widely used over 100 years, in which ammonia generated during decomposition is titrated. This classical method is not practical for a large number of samples as it requires the use of specially designed fragile and expensive glassware, and fume hoods because of the generation of toxic gases. We have developed a novel system designated the “Nitro-Ace” for the determination of the total nitrogen content of proteins and peptides using a colorimetric method in high-throughput technology. To realize routine analyses a novel reactor for decomposition of multiple samples was designed.


Total Nitrogen Content Fume Hood High Throughput Analysis Sodium Hypochloride Excess Hydrogen Peroxide 
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  1. 1.
    Kjeldahl, J. Z. Anal. Chem. 22, 366–382 (1883).CrossRefGoogle Scholar
  2. 2.
    Sugano, S., Fukui, S., Naito, S., Kaneko, M. Hygienic Chem. 14, 280–284 (1968).Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Kiyoshi Nokihara
    • 1
  • Tadashi Yasuhara
    • 2
  1. 1.Shimadzu Scientific Research Inc.KyotoJapan
  2. 2.Tokyo University of AgricultureTokyoJapan

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