Abstract
Determination of total nitrogen amounts is routinely carried out for the quality control of pharmaceutical products especially proteins and food products. For this purpose a method originally developed by Kjeldahl [1] has been widely used over 100 years, in which ammonia generated during decomposition is titrated. This classical method is not practical for a large number of samples as it requires the use of specially designed fragile and expensive glassware, and fume hoods because of the generation of toxic gases. We have developed a novel system designated the “Nitro-Ace” for the determination of the total nitrogen content of proteins and peptides using a colorimetric method in high-throughput technology. To realize routine analyses a novel reactor for decomposition of multiple samples was designed.
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References
Kjeldahl, J. Z. Anal. Chem. 22, 366–382 (1883).
Sugano, S., Fukui, S., Naito, S., Kaneko, M. Hygienic Chem. 14, 280–284 (1968).
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© 2001 Springer Science+Business Media Dordrecht
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Nokihara, K., Yasuhara, T. (2001). A Novel High Throughput Analysis of Total Nitrogen Content by a Colorimetric Method. In: Lebl, M., Houghten, R.A. (eds) Peptides: The Wave of the Future. American Peptide Symposia, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0464-0_129
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DOI: https://doi.org/10.1007/978-94-010-0464-0_129
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3905-5
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