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Large-scale plant proteomics

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Functional Genomics

Abstract

Large-scale and high throughput approaches increasingly play an essential role in the study of biological systems, which are per se highly complex. Therefore, they need to be examined by these extensive methods to receive information about the large genomic and proteomic networks. In plant biology, this purpose has a strong support through the accessability of the complete genome sequence of the model plant Arabidopsis thaliana. This brief review intends to focus on the basics and the state-of-the-art of these high-throughput technologies and their application to plant proteomics. It describes protein microarrays, the use of antibodies, 2-DE and MS methods and the yeast two hybrid system, which are emerging as the major technologies for plant proteomics.

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Authors and Affiliations

  1. Max Planck Institute of Molecular Genetics, Ihnestrasse 73, 14195, Berlin, Germany

    Birgit Kersten, Lukas Bürkle, Eckehard J. Kuhn, Patrick Giavalisco, Zoltan Konthur, Angelika Lueking & Ulrich Schneider

  2. biorchard AS, Nedre Skogvei 14, 0281, Oslo, Norway

    Birgit Kersten & Gerald Walter

  3. Scienion AG, Volmerstrasse 7a, 12489, Berlin, Germany

    Angelika Lueking, Holger Eickhoff & Ulrich Schneider

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  1. Birgit Kersten
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  2. Lukas Bürkle
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  3. Eckehard J. Kuhn
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  1. The Institute for Genomic Research, Rockville, MD, USA

    Chris Town

  2. Case Western Reserve University, Cleveland, OH, USA

    Chris Town

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Kersten, B. et al. (2002). Large-scale plant proteomics. In: Town, C. (eds) Functional Genomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0448-0_9

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  • DOI: https://doi.org/10.1007/978-94-010-0448-0_9

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