Ultrasonic Cell Separation — Production of Monoclonal Antibodies in Continuous Perfusion Cultures

  • Holger Heine
  • Christian Y. Arod
  • Alain R. Bernard
  • Horst D. Blasey
Chapter
Part of the ESACT Proceedings book series (ESACT, volume 1)

Abstract

The production of monoclonal andbodies (mABs) in small gram quanddes is mosdy done in batch or fed batch fermentations in stirred tank reactors. The use of perfusion systems tike hollow fibre or fluidized bed fermenters is considered as too laborious or not suitable in this scale. An alternative way is the use of a small continuously perfused stirred tank bioreactor with an ultrasonic cell setder (UCS) mounted on top of it as cell retention device. The cells are trapped in a standing wave ultrasonic field, which is switched on and off periodically to allow the cells to settle back into the fermenter. In contrast to other filtration devices like crossflow or microfilters the ultrasonic cell setder works without direct mechanical impact on the cells. This means that clogging of the device is avoided, which results in an excellent long-term stability.

Keywords

Ultrasonic cell separation perfusion cultures hybridoma mAB 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Trampler, F. Sonderhoff, S.A., Pui, RW.S., Kilbum, D.G., Piret, J.M.: “Acoustic Cell Filter for High Density Perfusion Culture of Hybridoma Cells”; Bio/Technology, 1994, 12, 281–284.PubMedCrossRefGoogle Scholar
  2. Sollner, K. and Bondy, C: “The mechanism of coagulation by ultrasonic waves”; Trans. Faraday Soc, 1936, 32, 616–623.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Holger Heine
    • 1
  • Christian Y. Arod
    • 1
  • Alain R. Bernard
    • 1
    • 3
  • Horst D. Blasey
    • 2
  1. 1.Serono Pharmaceutical Research InstituteSerono SAPlan-les-OuatesSwitzerland
  2. 2.Siegfried LtdZofingenSwitzerland
  3. 3.Serono, Zone IndustrielleCoinsinsSwitzerland

Personalised recommendations