Continuous Isolation of rFVIII from Mammalian Cell Culture

  • Jens H. Vogel
  • Markus Pritschet
  • Juergen Wolfgang
  • Paul Wu
  • Konstantin Konstantinov
Chapter
Part of the ESACT Proceedings book series (ESACT, volume 1)

Abstract

This is a proof-of-principle study on the potential application of continuous annular chromatography for the direct capture of protein product from perfusion culture supernatant, and the first step in its evaluation as potential platform technology for large scale production of recombinant protein drugs. A standard P-CAC column was specifically modified for isolation operation. Peak wobbling due to inhomogeneous flow was identified as a performance limiting factor. As a real world application, the continuous isolation of recombinant Factor VIII from culture supernatant using ion exchangeresin is presented. A sterile P-CAC prototype has been developed to allow on-line coupling to continuous perfusion fermenters.

Keywords

Fermentation Filtration Cabral 

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References

  1. Carta, G. and Byers, H. Separations by Continuous Annular Chromatography. C. A. Costa and J. S. Cabrai (eds.). Chromatographic and Membrane Processes in Biotechnology, pp. 101–114, Kluwer Academic Publisher, 1991.Google Scholar
  2. Vogel, J. H., Pritschet, M., Wolfgang, J., Wu, P. and Konstantinov, K. Continuous Annular Chromatography for Isolation of Recombinant Protein Drugs. Oral Presentation, 221th ACS National Meeting, San Diego, CA, April 1–5 2001.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Jens H. Vogel
    • 1
    • 3
  • Markus Pritschet
    • 2
  • Juergen Wolfgang
    • 2
  • Paul Wu
    • 1
  • Konstantin Konstantinov
    • 1
  1. 1.Bayer BiotechnologyCell Culture and Bioprocess EngineeringBerkeleyUSA
  2. 2.Prior Separation TechnologyGoetzisAustria
  3. 3.Bayer CorporationBerkeleyUSA

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