Application of an Acceleration-Stat to Study Insect Cell Metabolism
For over 30 years, various lines of insect cells have been cultured in semi-defined and serum-free media. However, litde is known about their metabolism. To obtain quantitative data for consumption and production rates of substrates and products, steady-state concentrations need to be determined. This requires the use of chemostat fermentations at various dilution rates. Due to the recommended stabilisation-time of three volume changes this is very time-consuming, especially at low dilution rates. Paalme et al. (1995) describes the use of the Acceleration-Stat as a fermentation system to obtain many steady-state data- point in a limited time. In an A-stat, at first a steady-state is obtained. After that, a smooth, computer-controlled change in dilution rate is applied.
KeywordsAcceleration-Stat insect cell metabolism glucose consumption chemostat
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