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Application of an Acceleration-Stat to Study Insect Cell Metabolism

  • Rolf Marteijn
  • Mariska Oude-Elferink
  • Remco Bron
  • Dirk Martens
  • Hans Tramper
Chapter
Part of the ESACT Proceedings book series (ESACT, volume 1)

Abstract

For over 30 years, various lines of insect cells have been cultured in semi-defined and serum-free media. However, litde is known about their metabolism. To obtain quantitative data for consumption and production rates of substrates and products, steady-state concentrations need to be determined. This requires the use of chemostat fermentations at various dilution rates. Due to the recommended stabilisation-time of three volume changes this is very time-consuming, especially at low dilution rates. Paalme et al. (1995) describes the use of the Acceleration-Stat as a fermentation system to obtain many steady-state data- point in a limited time. In an A-stat, at first a steady-state is obtained. After that, a smooth, computer-controlled change in dilution rate is applied.

Keywords

Acceleration-Stat insect cell metabolism glucose consumption chemostat 

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References

  1. Paalme-Toomas; Kahru-Anne; Elken-Raul; Vanatalu-Kalju and Tiisma-Kalle; Vilu-Raivo. The computer-controlled continuous culture of Escherichia coli with smooth change of dilution rate (A-stat). J. Microbiol. Methods 24(2) 145–153 (1995).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Rolf Marteijn
    • 1
  • Mariska Oude-Elferink
    • 1
  • Remco Bron
    • 1
  • Dirk Martens
    • 1
  • Hans Tramper
    • 1
  1. 1.Department of Agrotechnology and Food Sciences, Food and Bioprocess Engineering GroupWageningen UniversityEV Wageningen

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