Optimization of Medium for M2 (Anti-Flag) Hybridoma Using Factorial Matrix Designs
M2 hybridoma cehs secrete anti-FLAG antibody, used to detect and purify FLAG fusion proteins. Our goal was to develop a serum-free medium optimized for these cholesterol-dependent cells so that antibody production may be scaled up.
KeywordsCholesterol HPLC Cysteine Thymidine Transferrin
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