Optimization of Medium for M2 (Anti-Flag) Hybridoma Using Factorial Matrix Designs

  • S. Peppers
  • D. Allison
  • D. Talley
  • H. Loke
  • T. Johnson
  • M. Caple
Chapter
Part of the ESACT Proceedings book series (ESACT, volume 1)

Abstract

M2 hybridoma cehs secrete anti-FLAG antibody, used to detect and purify FLAG fusion proteins. Our goal was to develop a serum-free medium optimized for these cholesterol-dependent cells so that antibody production may be scaled up.

Keywords

Cholesterol HPLC Cysteine Thymidine Transferrin 

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References

  1. Peppers, S.C, D.W. Allison, T.K. Johnson, D.L. Talley, and M.V. Caple. “Replacing the Animal Component in Serum: Evaluating Raw Materials for Inclusion in Optimized Hybridoma Media.” Bio-Pharm 14.5 (May 2001): 22–30.Google Scholar
  2. Werner, R.G., “Balancing Financial Risk and Market Supply,” in Antibody Production and Downstream Processing, IBC’s 7th International Conference. January 31, 2001, International Business Communications, Westborough, MA.Google Scholar
  3. Myers, R.H., and D.C. Montgomery. Response Surface Methodology: Process and Product Optimization Using Designed Experiments. Wiley, New York, (p. 244) 1995.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • S. Peppers
    • 1
  • D. Allison
    • 1
  • D. Talley
    • 1
  • H. Loke
    • 1
  • T. Johnson
    • 1
  • M. Caple
    • 1
  1. 1.Sigma-Aldrich CorporationSt. LouisUSA

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