Optimization of Medium for M2 (Anti-Flag) Hybridoma Using Factorial Matrix Designs
M2 hybridoma cehs secrete anti-FLAG antibody, used to detect and purify FLAG fusion proteins. Our goal was to develop a serum-free medium optimized for these cholesterol-dependent cells so that antibody production may be scaled up.
KeywordsDesirability Function Spinner Flask Interaction Plot Viable Cell Density Factor Plot
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- Peppers, S.C, D.W. Allison, T.K. Johnson, D.L. Talley, and M.V. Caple. “Replacing the Animal Component in Serum: Evaluating Raw Materials for Inclusion in Optimized Hybridoma Media.” Bio-Pharm 14.5 (May 2001): 22–30.Google Scholar
- Werner, R.G., “Balancing Financial Risk and Market Supply,” in Antibody Production and Downstream Processing, IBC’s 7th International Conference. January 31, 2001, International Business Communications, Westborough, MA.Google Scholar
- Myers, R.H., and D.C. Montgomery. Response Surface Methodology: Process and Product Optimization Using Designed Experiments. Wiley, New York, (p. 244) 1995.Google Scholar