Abstract
M2 hybridoma cehs secrete anti-FLAG antibody, used to detect and purify FLAG fusion proteins. Our goal was to develop a serum-free medium optimized for these cholesterol-dependent cells so that antibody production may be scaled up.
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References
Peppers, S.C, D.W. Allison, T.K. Johnson, D.L. Talley, and M.V. Caple. “Replacing the Animal Component in Serum: Evaluating Raw Materials for Inclusion in Optimized Hybridoma Media.” Bio-Pharm 14.5 (May 2001): 22–30.
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© 2001 Springer Science+Business Media Dordrecht
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Peppers, S., Allison, D., Talley, D., Loke, H., Johnson, T., Caple, M. (2001). Optimization of Medium for M2 (Anti-Flag) Hybridoma Using Factorial Matrix Designs. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_5
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DOI: https://doi.org/10.1007/978-94-010-0369-8_5
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