The Role of Vitamins in Cell Culture Media
Cell culture media contain a multitude of components. In most cases a complete medium consists of a chemically well defined basal medium with additions of more or less defined additives. The basal medium contains low molecular weight substances such as inorganic ions, amino acids, vitamins and some other components (e.g. glucose, pyruvate, etc.). Usually, even an enriched basal medium is not suitable for the growth of mammalian cells in vitro. High molecular weight supplements, in particular proteins, have to be added to fulfil the requirements of the cells. Besides proteins (serum), these supplements may also contain peptides, lipoproteins, phospholipids or lipids. The group of vitamins is a Httle noticed target for media optimisation. Although the concentrations of these components are very low, the availability of vitamins is extremely important for the cells, since the cells usually cannot synthesise the vitamins. Vitamins act as co-factors for many enzymes and are essential for their function. The absence of vitamins in culture may lead to decrease in cell growth, cell death or loss of productivity.
KeywordsHeparin Pyruvate Aeration Thiamine Riboflavin
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