Abstract
The establishment of Chinese Hamster Ovary cells producing high affinity human recombinant anti RhD antibodies showing biological activity either by macrophage activation (chemiluminescence) and lysis of red blood cells by human lymphocytes (ADCC) has been published recently (1). To establish a process for the commercial production of human recombinant anti RhD antibodies, we studied pH as one of the most critical cell culture parameters. It was previously been reported the effects of lowering the pH on a generic fed-batch culture (3). In this study, two independent stable CHO cell lines expressing anti RhD antibodies (2) were compared with respect to the overall cell metabolism and productivity at pH values ranging from 6.9 up to 7.2 under batch conditions. The data we present confirms that addition of sodium ions in a form of NaCl raised product titers by 60 and 100% above those observed at respectively pH 7.2 and 6.9 for human recombinant Anti D producing CHO cell lines.
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Paul W. Sauer, John Burky, Marc Wesson, Heather Sternard, Limin Qu “A high-yielding, generic fed-batch cell culture process for production of recombinant antibodies” Biotechnology Bioengineering (2000), 585–597.
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© 2001 Springer Science+Business Media Dordrecht
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De Jesus, M.J. et al. (2001). The Influence of pH on Cell Growth and Specific Productivity of Two CHO Cell Lines Producing Human Anti Rh D IgG. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_43
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DOI: https://doi.org/10.1007/978-94-010-0369-8_43
Publisher Name: Springer, Dordrecht
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