Abstract
NSO cells has become increasingly used as host for production of therapeutics at large scale, therefore the adaptation to Serum and protein free media has been a priority for manufacturers. Gorfien et al, 2000, demonstrated that NS0 cells are unable to grow in CD hybridoma medium unless supplemented with cholesterol containing lipids emulsion. However, Keen and Steward, 1995, demonstrated that it is possible to adapt NS0 cells to protein free medium without cholesterol containing lipid supplementation and animal derived component, however the medium used it is quite complex for preparing and its use become almost impossible for industrial setting. Our results show that NS0 cell line can be adapted to grow in different commercially available protein free and animal component free media. Additionally, lipid supplementation was evaluated in order to know the impact of these on main metabolic parameter, specific MAb rate and the Viability Index.
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References
Gorphien Stephen, Paul B, Walowitz. J, Keen R, Biddle W and Jayme. D, 2000. Biotechnology Progress. Vol. 16.
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© 2001 Springer Science+Business Media Dordrecht
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Ojito, E., Labrada, G., Garcia, Z., Garcia, N., Chico, E. (2001). Study of NS0 Cell Line Metabolism in Lipid Supplemented Protein Free Media. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_38
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DOI: https://doi.org/10.1007/978-94-010-0369-8_38
Publisher Name: Springer, Dordrecht
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