Differential Enhancement of Monoclonal Antibody Production by Retinoids in Human Hybridomas
We examined enhancement of human monoclonal antibody (hMAb) production by retionids using five human hybridma cell lines. Retinyl acetate and retinoic acid (RA) enhanced hMAb production of the human hybridoma cell line AD2, AE6 and BD9 but not HB4C5 and HF10B4. These findings implied that enhancement by retinoids may be influenced by the fusion partner of human hybridoma cell line rather than immunoglobulin isotypes produced. When the expression levels of retinoid receptors (RARs and RXRs) were compared among hybridoma cell lines treated with RA, that of RXR-alpha was found to be remarkably low in both HB4C5 and HF10B4 cells. This low expression level of RXR-alpha was found in the fusion partner of HB4C5 and HF10B4 cells, NAT-30. These results show that the differential enhancement of hMAb production by retinoids may be, in part, related to RXR-alpha gene expression level of human hybridoma cell line which seemed to be inherited from its fusion partner.
KeywordsTransferrin Selenite Retinoid Ethanolamine Retinyl
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