Differential Enhancement of Monoclonal Antibody Production by Retinoids in Human Hybridomas

  • Yuichi Inoue
  • Sanetaka Shirahata
Part of the ESACT Proceedings book series (ESACT, volume 1)


We examined enhancement of human monoclonal antibody (hMAb) production by retionids using five human hybridma cell lines. Retinyl acetate and retinoic acid (RA) enhanced hMAb production of the human hybridoma cell line AD2, AE6 and BD9 but not HB4C5 and HF10B4. These findings implied that enhancement by retinoids may be influenced by the fusion partner of human hybridoma cell line rather than immunoglobulin isotypes produced. When the expression levels of retinoid receptors (RARs and RXRs) were compared among hybridoma cell lines treated with RA, that of RXR-alpha was found to be remarkably low in both HB4C5 and HF10B4 cells. This low expression level of RXR-alpha was found in the fusion partner of HB4C5 and HF10B4 cells, NAT-30. These results show that the differential enhancement of hMAb production by retinoids may be, in part, related to RXR-alpha gene expression level of human hybridoma cell line which seemed to be inherited from its fusion partner.


Retinoic Acid Fusion Partner Retinoid Receptor Monoclonal Antibody Production Retinyl Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aotsuka, Y. and Naito, M. (1991) Enhancing effects of retinoic acid on monoclonal antibody production of human-human hybridomas, Cell. Immunol. 133, 498–505.PubMedCrossRefGoogle Scholar
  2. Heyman, R.A., Mangelsdorf, D.J., Dyck, J.A., et al. (1992) 9-Cis rednoic acid is a high affinity ligand for the retinoid X receptor. Cell 68, 397–406.PubMedCrossRefGoogle Scholar
  3. Inoue, Y, Fujisawa, M., Shoji, M., et al. (2000) Enhanced antibody production of human-human hybridomas by retinoic acid, Cytotechnology 33, 83–88.PubMedCrossRefGoogle Scholar
  4. Murakami, H., Hashizume, H., Ohashi, H., et al. (1985) Human-human hybridoma secreting antibodies specific to human lung carcinoma. In Vitro Cell. Dev. Biol. 21, 593–596.PubMedCrossRefGoogle Scholar
  5. Nugent, R, Sucov, H.M., Pisano, M.M., et al. (1999) The role of RXR-alpha in retinoic acid-induced cleft palate as assessed with the RXR-alpha knockout mouse, Int. J. Dev. Biol. 43, 567–570.PubMedGoogle Scholar
  6. Shoji, M., Kawamoto, S., Seki, K., et al. (1996) Lung cancer-reacting human recombinant antibody AE6F4: potential usefulness in the sputum cytodiagnosis, Hum. Antibod. Hybridoma 7, 27–36.Google Scholar
  7. Wu, S., Zhang, Z., Zhang, D., et al. (1997) Reduction of both RAR and RXR levels is required to maximally alter sensitivity of CA-0V3 ovarian tumor cells to growth suppression by all-trans-retinoic acid, Exp. Cell Res. 237, 118–126.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • Yuichi Inoue
    • 1
  • Sanetaka Shirahata
    • 2
  1. 1.Faculty of AgricultureKagoshima UniversityKagoshimaJapan
  2. 2.Graduate School of Genetic Resources TechnologyKyushu UniversityFukuokaJapan

Personalised recommendations