Abstract
The unstabihty transfected cells (Kim et al, 2001; Kim et al, 1996; Morrinson et al, 1997) has been studied by several groups. These investigations have shown a general decline in antibody secretion level over long-term culture. It is, generally, due to the appearance of non-producer (NP) or low producer (LP) population. The decrease in antibody productivity is irreversible. Morrinson et al. 1997, showed the reversibility of expression loss in transfected cells, suggest the methylation as a cause of this kind of behavior.
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References
Kim, J. H., Bae, S. W., Hong, H. J., Lee, G. M. Decreasing chimeric productivity of KR12H-1 transfectoma during long-term culture result from decreased of antibody gene copy number. Biotechnol. Bioeng. 51, (1996): 479–487.
Kim, N. S., Byun, T. H., Lee, G. M. Key determinants in the occurrence of clonal variation in humanized antibody expression of CHO cells during dihydrofolate reductase mediated gene amplification. Biotechnol. Prog. 17, (2001): 69–75.
Morrinson, C. J., McMaster, W. R., Piret, J. M. Differential stability of Proteolytically active and inactive recombinant metalloproteinase in Chinese hamster ovary cells. Biotechnol. Bioeng. 53 (1997): 594–600.
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© 2001 Springer Science+Business Media Dordrecht
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Boggiano, T. (2001). Loss of Productivity of a Transfectoma in Long-Term Culture: Recovery of Expression. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_32
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DOI: https://doi.org/10.1007/978-94-010-0369-8_32
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