Abstract
The increasing demand of milUgram amounts of recombinant proteins for high throughput screening forces the development of fast and efficient expression systems. Mammalian expression systems have the advantage of yielding proteins with posttranslational modifications, which is an important feature when using proteins in animal models. Besides the classical cell lines hke CHO or COS the human embryonic kidney cell line HEK293-EBNA is more and more commonly used. This expression system offers the possibiUty to produce recombinant proteins with human glycosylation pattern. This generates less false positives in the screening and therefore saves time and money.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
M. Jordan, A. Schallhorn and F.M. Wurm: “Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation’, Nucleic Acids Research, 1996, 24, 596–601.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Heine, H., Solazzo, MJ.F., Rossi, C.P., Papoian, R., Bernard, A.R., Blasey, H.D. (2001). In 30 Days from Plasmid to Product: Expression of a Secreted Fusion Protein Using Hek293-EBNA Cells. In: Lindner-Olsson, E., Chatzissavidou, N., Lüllau, E. (eds) Animal Cell Technology: From Target to Market. ESACT Proceedings, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0369-8_20
Download citation
DOI: https://doi.org/10.1007/978-94-010-0369-8_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3897-3
Online ISBN: 978-94-010-0369-8
eBook Packages: Springer Book Archive