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Capillary-Aided Cell Cloning

A Technique for One Step Cloning with High Probability of Monoclonality
  • A. O. Onadipe
  • H. K. Metcalfe
  • P. R. Freeman
  • C. James
Chapter
Part of the ESACT Proceedings book series (ESACT, volume 1)

Abstract

Capillary-aided cell cloning relies on independent visual confirmation by two scientists of the presence of a single cell in a 1 μl droplet. This technique has been shown to permit the resolution of a mixed cell culture to give monoclonal colonies. The high probability of monoclonality obtained using this technique means that only one round of cloning is required, resulting in time and cost savings in cell line development for therapeutic protein production. This technique has been used to clone hybridoma, CHO and NS0 cell lines.

Keywords

Cloning monoclonality NS0 CHO hybridoma glutamine synthetase 

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Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • A. O. Onadipe
    • 3
  • H. K. Metcalfe
    • 3
  • P. R. Freeman
    • 1
  • C. James
    • 2
  1. 1.Ship CottageHelston, CornwallUK
  2. 2.RSS Centre for Statistical EducationNottingham Trent UniversityNottinghamUK
  3. 3.Lonza Biologics plcSlough, BerkshireUK

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