Continuous Retroviral Vector Production in a Perfusion Bioreactor System
A successful application of retroviral vectors for gene therapy depends on high levels of gene transfer into target cells. Therefore large amounts of high titre retroviral supernatants are required. The most commonly used systems for the generation of retroviral supernatant are T-flasks or roller bottles. The major problems of these conventional production systems are the limited yield of infectious retroviral particles, due to the short production-time and the instability of the infectious viral particles at 37°. To overcome these limitations we established a perfusion bioreactor system with macroporous microcarriers for the growth of adherent producer cell lines. Perfusion bioreactor systems allow the continuous production of retroviral supernatant at constant substrate concentrations and the storage of supernatant at 4° to increase the stability of infectious retroviral particles.
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