Abstract
A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescent in situ hybridization has been applied to analyze the location and organization of five different highly-repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin has different properties with regard to anchorage to the SC. While the organization of telomere sequences is maintained in different organisms, species seem to differ with respect to the organization of centromeric satellites.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Abbo, S., Dunford, R.P., Foote, T., Reader, S.M., Flavell, R.B. and Moore, G. (1995) Organization of retroelement and stem-loop repeat families in the genomes and nuclei of cereals. Chromosome Res. 3
Aragón-Alcaide, L., Miller, T., Schwarzacher, T., Reader, S. and Moore, G. (1996) A cereal centromeric sequence. Chromosoma 105:261-268.
Barlow, A.L. and Hultén, M.A. (1996) Combined immunocytogenetic and molecular cytogenetic analysis of meiosis I human spermatocytes. Chromosome Res. 4:562-573.
Bedbrook, J.R., Jones, J., ÓDell, M., Thompsom, R.D. and Flavell, R.B. (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545-560.
Cox, A.V., Bennett, S.T., Parokonny, A.S., Kenton, A., Callimassia, M.A. and Bennett, M.D. (1993) Comparison of plant telomere locations using PCR-generated synthetic probe. Ann. Bot. 72:239-247.
Cuadrado, A., Ceoloni, C. and Jouve, N. (1995) Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization. Genome. 38:1061-1069.
Cuñado, N., Garrido-Ramos, M.A., de la Herran, R., Ruiz Rejon. C., Ruiz Rejob. M., and Santos, J.L. 2000. Organization of repetitive DNA sequences at pachytene chromosomes of gilthead seabream Sparus aurata (Pisces, Perciformes). Chromosome Res. 8: 67-72.
Cuñado, N. and Santos, J.L. (1998) A method for fluorescence in situ hybridization against synaptonemal complex-associated chromatin of plant meiocytes. Exp. Cell Res. 239:179-182.
Fuchs, J., Brandes A and Schubert, I., (1995) Telomere sequence localization and karyotype evolution in higher plants. Plant Syst. Evol. 196:227-241.
Gerlach, W.L. and Bedbrook, J.R. (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic. Ac. Res. 7:1869-1885.
Heng HHQ, Tsui L-C and Moens, P.B. (1994) Organization of heterologous DNA inserts on-the mouse meiotic chromosome core. Chromosoma 103:401-407
Heng, H.H.Q., Chamberlain, J.W., Shi X-M, Spyropoulos, B., Tsui L-C and Moens, P.B. (1996) Regulation of meiotic chromatin loop size by chromosomal position. Proc. Nat. Acad. Sci. USA 93:2795-2800.
Holm, P.B. (1986) Chromosome pairing and chiasma formation in allohexaploid wheat, Triticum aestivum, analysed by spreading of meiotic nuclei. Carlsberg Res. Commun. 51:239-294.
Moens, P.B. and Pearlman, R.E. (1988) Chromatin organization at meiosis. Bioessays 9:151-153.
Moens, P.B. and Pearlman, R.E. (1989) Satellite DNA I in chromatin loops of rat pachytene chromosomes and in spermatids, Chromosoma 98:287-294.
Moens, P.B. and Pearlman, R.E. (1990a) In situ DNA sequence mapping with surface-spread mouse pachytene chromosomes, Cytogenet. Cell Genet. 53: 219-220.
Moens, P.B. and Pearlman, R.E. (1990b) Telomere and centromere DNA are associated with the cores of meiotic prophase chromosomes. Chromosoma 100:8-14.
Peterson, D.G., Lapitan, N.L.V. and Stack, S.M. (1999) Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics 152:427- 439.
Sandery, M.J., Forster, J.W., Blunden, R. and Jones, R.N. (1990) Identification of a family of repeated sequences on the rye B-chromosome. Genome. 34:88-95.
Solari, A.J. and Dresser, M.E. (1995) High-resolution cytological localization of the XhoI and EcoRI repeat sequences in the pachytene, Z.W. bivalent of the chicken. Chromosome Res. 3:87-93.
Weith, A. and Traut, W. (1980) Synaptonemal complexes with associated chromatin in a moth, Ephestia kuehniella, Z Chromosoma 78:275-291.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Narosa Publishing House, New Delhi, India
About this chapter
Cite this chapter
Cuñado, N., Barrios, J., Santos, J.L. (2002). Mapping and Organization of Highly-Repeated Sequences by Means of Fluorescence in situ Hybridization Against Synaptonemal Complex-Associated Chromatin of Rye. In: Sobit, R.C., Obe, G., Athwal, R.S. (eds) Some Aspects of Chromosome Structure and Functions. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0334-6_2
Download citation
DOI: https://doi.org/10.1007/978-94-010-0334-6_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-7057-4
Online ISBN: 978-94-010-0334-6
eBook Packages: Springer Book Archive