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Chromosome painting as a tool for rice genetics and breeding

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Chromosome Painting

Abstract

Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.

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Abbreviations

McGISH:

Multi-color genomic in situ hybridization

FISH:

Fluorescence in situ hybridization

RIRE1:

Rice retrotransposon 1

RFLP:

Restriction fragment length polymorphism

TrsA:

Tandem repeat sequence A

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Author information

Authors and Affiliations

  1. Department of Plant Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-8510, Japan

    Rieko Shishido

  2. Laboratory of Rice Genetic Engineering, National Agricultural Research Center, Hokuriku Center, Joetsu, 943-0193, Japan

    Nobuko Ohmido

  3. Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan

    Kiichi Fukui

Authors
  1. Rieko Shishido
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  2. Nobuko Ohmido
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  3. Kiichi Fukui
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Editor information

Editors and Affiliations

  1. Centre for Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcultta, Calcultta, India

    Arun Kumar Sharma  & Archana Sharma  & 

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© 2001 Springer Science+Business Media Dordrecht

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Shishido, R., Ohmido, N., Fukui, K. (2001). Chromosome painting as a tool for rice genetics and breeding. In: Sharma, A.K., Sharma, A. (eds) Chromosome Painting. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0330-8_12

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  • DOI: https://doi.org/10.1007/978-94-010-0330-8_12

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3840-9

  • Online ISBN: 978-94-010-0330-8

  • eBook Packages: Springer Book Archive

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