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Microdissection and chromosome painting of plant B chromosomes

  • Chapter
Chromosome Painting

Abstract

Plant chromosome microdissection techniques together with different isolation and amplification methods of microisolated DNA are described. Such isolated DNA was used to ‘chromosome paint’ B chromosomes of the dicot Brachycome dichromo-somatica and the monocot Secale cereale. It is demonstrated that the specific painting of the described chromosomes was possible because of enrichment for chromosome-specific repetitive sequences, rather than the chromosome specific low-and single-copy sequences which are responsible for the painting of mammalian chromosomes. The feasibility of ‘chromosome painting’ of standard chromosomes in plant species with relatively small or large genomes is discussed.

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Authors and Affiliations

  1. Department of Genetics, The University of Adelaide, South Australia, 5005, Australia

    Andreas Houben, Bruce L. Field & Verity A. Saunders

Authors
  1. Andreas Houben
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  2. Bruce L. Field
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  3. Verity A. Saunders
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Correspondence to Andreas Houben .

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Editors and Affiliations

  1. Centre for Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcultta, Calcultta, India

    Arun Kumar Sharma  & Archana Sharma  & 

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Houben, A., Field, B.L., Saunders, V.A. (2001). Microdissection and chromosome painting of plant B chromosomes. In: Sharma, A.K., Sharma, A. (eds) Chromosome Painting. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0330-8_11

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  • DOI: https://doi.org/10.1007/978-94-010-0330-8_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3840-9

  • Online ISBN: 978-94-010-0330-8

  • eBook Packages: Springer Book Archive

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