Abstract
We present a batch microfermentor with a working volume of 5 μℓ, with integrated sensors for the measurement of dissolved oxygen (DO) and pH. Growth of Escherichia coli cells is demonstrated in the batch microfermentors. The biomass is determined using measurements of optical density (OD). Growth behavior as well as the curves of DO and pH are comparable to results obtained from conventional 500 mℓ batch fermentors.
These authors contributed equally to this work
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References
A. Zanzotto, N. Szita, Martin A. Schmidt, Klavs F. Jensen, Microfermentors for the rapid screening and analysis of biochemical processes, Proc. IEEE-EMBS 2002, pp. 164–168,(2002).
D.C. Duffy, J. Cooper McDonald, O.J.A. Schueller, G.M. Whitesides, Rapid prototyping of microfluidic systems in poly(dimethylsiloxane), Anal. Chem., Vol. 70, No. 23, pp. 4974–4984, (1998).
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© 2002 Springer Science+Business Media Dordrecht
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Szita, N., Zanzotto, A., Boccazzi, P., Sinskey, A.J., Schmidt, M.A., Jensen, K.F. (2002). Monitoring of Cell Growth, Oxygen and pH in Microfermentors. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0295-0_2
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DOI: https://doi.org/10.1007/978-94-010-0295-0_2
Publisher Name: Springer, Dordrecht
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