Abstract
We demonstrate the passive capture of a single cell in a specific area of a microfluidic chip and the active delivery of reagents or compounds to that cell. This delivery of reagents and the wash steps are very rapid. The chip can be used to perform many different types of cell-based assays, such as Ca++ flux and apoptosis. Both mix-and-measure and heterogeneous assays can be performed in real-time. The trap can be used serially for multiple assays, because a trapped cell can be dispensed after the assay either to waste or to a downstream function, such as a region for further culturing.
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References
M.A. Unger, et al., Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography, Science, pp.113–116, (2000).
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© 2002 Springer Science+Business Media Dordrecht
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Daridon, A. et al. (2002). A Programmable Cell Assay Platform for Kinetic Studies of a Single Cell. In: Baba, Y., Shoji, S., van den Berg, A. (eds) Micro Total Analysis Systems 2002. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0295-0_10
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DOI: https://doi.org/10.1007/978-94-010-0295-0_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3952-9
Online ISBN: 978-94-010-0295-0
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