APH Induced Red Cell Oxidation in Healthy Persons

Abstract

In a previous work we have shown that the red blood cells of patients suffering from breast cancer (P) seem more sensitive to the denaturing action of acetylphenylhydrazine (APH) than those from healthy donors (H). The amount and the formation time of the hemin, one of the last oxidation products, are significantly different in the patients. Therefore, the spectral contribution of hemin after the same incubation time could be a factor of discrimination between the samples from the patients and healthy population. The aim of the present work was to gain a deeper understanding of the oxidation processes and establish a standard setup for the measurements.