Nonenzymic Degradation and Decolorization of Recalcitrant Compounds

  • F. Nerud
  • P. Baldrian
  • J. Gabriel
  • D. Ogbeifun
Part of the NATO Science Series book series (NAIV, volume 19)


The ability of a nonenzymic system containing CuII/pyridine/peroxide to decolorize structurally different synthetic dyes and to degrade selected PAHs was followed. An intense and rapid (after 1 h) decolorization has been obtained with phenol red (89%), Evans blue (95%) eosin yellowish (84%) and Poly B-411 (92%). The use of radical scavengers, thiourea and Superoxide dismutase, showed that hydroxyl radicals rather than Superoxide anions are involved in the decolorization. The intensive degradation of PAHs has been obtained after 24 h. Benzo[a]pyrene has also been degraded by the CuII/H2O2 peroxide system.


Evans Blue Ligninolytic Enzyme Cumene Hydroperoxide Fenton Reagent Peroxide System 
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  1. 1.
    Hamby, D.M. (1996) Site remediation techniques supporting environmental restoration activities — a review, Sci. Total Environ. 191, 203–224.CrossRefGoogle Scholar
  2. 2.
    Cerniglia, C.E. (1992) Biodegradation of polycyclic aromatic hydrocarbons, Biodegradation 3, 351–368.CrossRefGoogle Scholar
  3. 3.
    Paszynski, A. and Crawford, R.L. (1995) Potential for bioremediation of xenobiotic compounds by the white rot fungus Phanerochaete chrysosporium, Biotechnol. Prog. 11, 368–379.CrossRefGoogle Scholar
  4. 4.
    Banat, I.M., Nigam, P., Singh, D., and Marchant, R. (1996), Microbial decolorization of textile dye containing effluents: A review, Bires. Technol. 58, 217–227.CrossRefGoogle Scholar
  5. 5.
    Field, J.A., de Jong, E., Feijoo-Costa, G., and de Bont J.A.M. (1993) Screening for ligninolytic fungi aplicable to the biodegradation of xenobiotics, Trends Biotechnol. 11, 44–49.CrossRefGoogle Scholar
  6. 6.
    Nerud, F., Zouchová, Z., and Mišurcová, Z. (1991) Ligninolytic properties of different white rot fungi, Biotechnol. Lett. 13, 657–660.CrossRefGoogle Scholar
  7. 7.
    Hatakka, A. (1994) Lignin-modifying enzymes from selected white rot fungi: production and role in lignin degradation, FEMS Microbiol Reu. 13, 125–135.CrossRefGoogle Scholar
  8. 8.
    Nerud, F. and Mišurcová, Z. (1996) Distribution of ligninolytic enzymes in selected white rot fungi, Folia Microbiol. 41, 264–266.CrossRefGoogle Scholar
  9. 9.
    Bezalel, L., Hadar, Y., and Cerniglia, C.E. (1996) Mineralization of polycyclic aromatic hydrocarbons by the white rot fungus Pleurotus ostreatus, Appl. Environ. Microbiol. 62, 292–295.Google Scholar
  10. 10.
    Nerud, F., Homolka, L., Eichlerová, I., and Gabriel, J. (1999) Biodegradative ability of Pleurotus ostreatus isolates overproducing ligninolytic enzymes. The 5th International Symposium In situ on site Bioremediation, Abstract Book (posters) B6, April 19–22, San Diego, California.Google Scholar
  11. 11.
    Koenigs, J.W. (1974) Hydrogen peroxide and iron: A proposed system for decomposition of wood by brown rot basidiomycetes, Wood Fiber Sci. 6 66–80.Google Scholar
  12. 12.
    Guillén, F., Martínez, M.J., Munoz, C., and Martínez, A.T. (1997)Quinone redox cycling in the ligninolytic fungus Pleurotus eryngii leading to extracellular production of Superoxide aninon radical, Arch. Biochem. Biphys. 339, 190–197.CrossRefGoogle Scholar
  13. 13.
    Kotterman, M.J., Wasseveld, R.A., and Field, J.A. (1996) Hydrogen peroxide as limiting factor in xenobiotic oxidation by nitrogen-sufficient cultures of Bjerkandera sp. strain BOS55 overproducing peroxidases, Appl. Environ. Microbiol. 62, 880–885.Google Scholar
  14. 14.
    Wood, P.M. (1994) Pathways for production of Fenton’s reagent by wood rotting fungi, FEMS Microbiol Reu. 13, 313–320.CrossRefGoogle Scholar
  15. 15.
    Barbeni, M., Minero, C., and Pellizetti(1987): Chemical degradation of chlorophenols with Fenton’s reagent. Chemosphere 16, 2225–2237.CrossRefGoogle Scholar
  16. 16.
    Ollis, D.F. and Al-Akabi, H. (1993): Photocatalytic Purification and Treatment of Water and Air, Elsevier, Amsterdam.Google Scholar
  17. 17.
    Herrera, F., Kiwi, A., Lopez, A., and Nadtochenko, V. (1999): Photochemical decoloration of remazol brilliant blue and uniblue A in the presence of Fe3+ and H2O2. Environ. Sci. Teclmol. 33, 3145–3151.CrossRefGoogle Scholar
  18. 18.
    Watanabe, T., Koller, K., and Messner, K. (1998) Copper-dependent depolymerization of lignin in the presence of fungal metabolite pyridine. J. Biotechnol. 62, 221–230.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  • F. Nerud
    • 1
  • P. Baldrian
    • 1
  • J. Gabriel
    • 1
  • D. Ogbeifun
    • 1
  1. 1.Laboratory of Biochemistry of Wood-Rotting Fungi Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague, CzechiaCzech Republic

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