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Micropropagation of Tropical Woody Species

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Micropropagation of Woody Trees and Fruits

Part of the book series: Forestry Sciences ((FOSC,volume 75))

Abstract

During the last thirty years plant micropropagation has been one of the most significant contributions of biotechnology to the horticultural industry (Giles and Friesen, 1994). The possibility of mass propagation of elite, endangered, or difficult-to-propagate genotypes, in relatively small areas and time period, is the most important characteristic of micropropagation. In association with breeding programs micropropagation can have a significant impact not only in the multiplication of important genetic material, but also for thein vitromaintenance of germplasm.

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References

  • Aguiar Filho S.P., Bosco J.A 1995 Mangabeira e sua importancia para o tabuleiro costeiro paraibano. Informativo SBF, Itajaí, v. 14, no 4. 26p.

    Google Scholar 

  • Ajithkumar D., Seeni S. 1998 Rapid clonal multiplication throughin vitroaxillary shoot proliferation ofAegle marmelos(L.) Corr., a medicinal tree. Plant Cell Rep 17:422–426.

    Article  CAS  Google Scholar 

  • Anand A., Rao C.S., Balakrishna P. 1999In vitropropagation ofSyzygium travancoricumGamble — an endangered tree species. Plant Cell Tiss Org Cult 56:59–63.

    Article  Google Scholar 

  • Antonetti P.L.E., Pinon J. 1993 Somaclonal variation within poplar. Plant Cell Tiss Org Cult 35:99–106.

    Article  Google Scholar 

  • Bajaj Y.P.S. 1990 Somaclonal variation - origin, induction, cryopreservation, and implications in plant breeding. In: Bajaj Y.P.S. (ed) Somaclonal variation in crop improvement I. Springer-Verlag, Berlin. pp. 3–48.

    Chapter  Google Scholar 

  • Barrueto Cid L.P., Gomes A.C.M., Costa S.B.R., Teixeira, J.B. 1997 Micropropagation ofMiconiasp, a woody Melastomaceae from Brazil, using thidiazuron as plant growth regulator. R Bras Fisiol Veg 9:21–25.

    CAS  Google Scholar 

  • Brown P.T.H., Lange F.D. Kranz E, Lorz H. 1993 Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol Gen Genet 237:311–317

    PubMed  CAS  Google Scholar 

  • Campinhos E., Ikemori Y.K. 1983 Introduçao de novas técnicas na produçao de mudas deessências florestais. Silvicultura 8:226–228.

    Google Scholar 

  • Carron M.P., Le Roux Y., Tison J., Dea B.G., Caussanel V., Clair J., Keli J. 2000 Compared root system architectures in seedlings andin vitroplantlets ofHevea brasiliensisin the initial years of growth in the field. Plant and Soil 223:73–85.

    Article  CAS  Google Scholar 

  • Cato S., Richardson T.E. 1996 Development and evaluation of AFLPs inPinus radiata.Plant Genome IV Program, 69. International Plant Genome Conference, January 14–18, 1996. San Diego, CA.

    Google Scholar 

  • Cecchini E., Natali L., Cavallini A., Durante M. 1992 DNA variations in regenerated plants of pea(Pisum sativumL.). Theor Appl Genet 84:874–879.

    Article  CAS  Google Scholar 

  • Cloutier S., Landry B.S. 1994 Molecular markers applied to plant tissue culture. In Vitro Cell Dev Biol - Plant 30P:32–39.

    Google Scholar 

  • Correia D. 1993 Crescimento e desenvolvimento de gemas na multiplicaçáo de Eucalyptus sppin vitroem meïo de cultura líquido e sólido. MS Thesis, ESALQ, University of São Paulo, Piracicaba, 113p.

    Google Scholar 

  • Correia D., Gonçalves A.N., Couto, H.T.G. do, Ribeiro, M.C. 1995 Efeito do meio decultura líquido e sólido no crescimento e desenvolvimento de gemas deEucalyptus grandisxEucalyptus urophyllana multiplicaçáoin vitro.IPEF, 48/49:107–116.

    Google Scholar 

  • De Klerk G.-J. 1990 How to measure somaclonal variation. Acta Bot Neerl 39:129–144.

    Google Scholar 

  • De Fossard R.A. 1974 Tissue culture ofEucalyptus.Australian Forestry 37:43–54.

    Article  CAS  Google Scholar 

  • Detrez C., Ndiaye S., Dreyfus B. 1994In vitroregeneration of the tropical multipurposeleguminous treeSesbania grandiflorafrom cotyledon explants. Plant Cell Rep 14:87–93

    Google Scholar 

  • Deverno L.L. 1995 An evaluation of somaclonal variation during somatic embryogenesis. In: Jain S.M., Gupta P., Newton R. (eds.) Somatic embryogenesis in woody plants. Kluwer Academic Publishers. Dordrecht. Vol. 1, pp. 361–377.

    Google Scholar 

  • Dornelas M.C., Vieira M.L. 1994 Tissue culture studies on species ofPassiflora.Plant Cell Tiss Org Cult 36:211–217.

    Article  CAS  Google Scholar 

  • Drew R.A. 1991In vitroculture of adult and juvenile explants ofPassifloraspecies. Plant Cell Tiss Org Cult 26:23–27.

    Article  Google Scholar 

  • D’ Souza M.C., Sharon, M. 2001 In vitro clonal propagation of annatto (Bixa orellana L.). In Vitro Cell Dev Biol Plant 37:168–172.

    Article  Google Scholar 

  • Faria J.L.C., Segura J. 1997aIn vitrocontrol of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants ofPassiflora edulisf.flavicarpa. In VitroCell Dev Biol - Plant 33:209–212.

    Article  CAS  Google Scholar 

  • Faria J.L.C., Segura J. 1997b Micropropagation of yellow passion fruit by axillary bud proliferation. HortSci 32:1276–1277.

    Google Scholar 

  • Faye C., Murigneux A., Bonjean A., Lacaze P., Peleman J. 1996 AFLPTM mapping in maize:distribution and stability of the bands. Plant Genome IV, 73. International Plant Genome Conference. January 14–18, 1996. San Diego, CA.

    Google Scholar 

  • Fourré J.-L., Berger P., Niguel L., André P. 1997 Somatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approaches. Theor Appl Genet 94:159–169.

    Article  Google Scholar 

  • Gabryszewska E., Rudnicki R.M., Blacquiere T., Gude H. 1997 The effects of light quality on the growth and development of shoots and roots ofFicus benjamina in vitro.Acta Hort 418:163–167.

    Google Scholar 

  • George E.F. 1993 Plant propagation by tissue culture, part 1 - The technology. Exegetics Ltd, Edington, Wilts, England. pp. 67–87; 261–263.

    Google Scholar 

  • George E.F., Sherrington P.D. 1984 Plant propagation by tissue culture: handbook and directory of commercial laboratories. Exegetics, Eversley. 709p.

    Google Scholar 

  • Giles K.L., Friesen K.R.D. 1994. Micropropagation. In. Shargool, P.D and Ngo, T.T. (eds.) Biotechnologial applications of plant cell cultures. CRC Press. Boca Raton. p. 111–128.

    Google Scholar 

  • Gonçalves A.N. 1980. Reversion to juvenility and cloning ofEucalyptus urophyllaS. T. Blake in cell and tissue culture system. In. Simpósio IUFRO em melhoramento genético e produtividade de espécies florestais de räpido crescimento. Aguas de Sao Pedro, SP. Brazil. p. 15–30.

    Google Scholar 

  • Grattapaglia D., Machado M.A. 1990 Micropropagaçáo. In: Torres, A.C.; Caldas, L.S. Técnicas e aplicaçóes da cultura de tecidos de plantas. ABCTP/EMBRAPA - CNPH. 433p.

    Google Scholar 

  • Grattapaglia D.. Caldas, L.S., Silva J.R. da, Machado M.A. 1991 Cultura de tecidos de maracujá. In. San José A.R., Ferreira, F.R., Vaz. R.L. (eds.). A cultura do maracujá no Brasil. FUNEP, Jaboticabal. p. 61–77.

    Google Scholar 

  • Gresshoff P.M., Doy C.H. 1972 Development of differentiation of haploidLycopersicon esculentum(tomato). Planta 107: 161–170.

    Article  Google Scholar 

  • Grossi F. 1995 Adequaçáo nutricional do meio de cultura para crescimento e desenvolvimento de gemas de Eucalyptus saligna Smith in vitro, procedência Itatinga. MS thesis. ESALQ, University of Sao Paulo, Piracicaba. 125p.

    Google Scholar 

  • Gupta P.K., Timmis R., Mascarenhas A.F. 1991 Field performance of micropropagated forestry species.In VitroCell Dev Biol - Plant 27P:159–164.

    Article  Google Scholar 

  • Hammerschlag, F.A. 1992 Somaclonal variation. In: Hammerschlag F.A., Litz R.E. (eds). Biotechnology of perennial fruit crops. CAB International, Wallingford, Vol 8 pp. 35–36.

    Google Scholar 

  • Hammerschlag F.A., Scorza R. 1991 Field performance of micropropagated, own-rooted peach trees. J Amer Soc Hort Sci 116:1089–1091.

    Google Scholar 

  • Hashmi G., Huettel R., Meyer R., Krusberg L., Hammerschlag F. 1997 RAPD analysis of somaclonal variants derived from embryo callus cultures of peach. Plant Cell Rep 16:624–627.

    Article  CAS  Google Scholar 

  • Heinz D.J.. Mee G.W.Y., Nickell L.G. 1969 Chromosome number of someSaccharumspecies hybrids and their cell suspension cultures. Am J Bot 56:450–456.

    Article  Google Scholar 

  • Heinz D.J., Mec G.W.P. 1971 Morphologic, cytogenetic, and enzymatic variation inSaccharumspecies hybrid clones derived from callus tissue. Am J Bot 58:257–262.

    Article  Google Scholar 

  • Higashi E.N., Silveira R.L.V.A., Gonçalves. A.N. 2000 Evoluçäo do jardim clonal de eucalipto para a produçáo de mudas. IPEF Noticias 24:4–6.

    Google Scholar 

  • Hill M., Witsenboer H., Zabeau M., Vos P., Kesseli R., Michelmore R. 1996 PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships inLactucaspp. Theor Appl Genet 93:1202–1210.

    Article  CAS  Google Scholar 

  • Huang L.C., Huang B.L., Wang C.H., Kuo C.I., Murashige T. 2000 Developing an improvedin vitropropagation system for slow-growing species usingGarcinia mangostaeaL. (mangosteen).In VitroCell Dev Biol - Plant 36:501–504.

    Article  Google Scholar 

  • Iannelli C., Xavier A., Comério J. 1996 Micropropagaçdo deEucalyptusspp na Champion. Silvicultura 17:33–35.

    Google Scholar 

  • Isabel N., Tremblay L., Michaud M., F.M. Tremblay F.M., Bousquet J. 1993 RAPDs as an aid to evaluate the genetic integrity of somatic embryo-derived populations ofPicea mariana(Mill.) B.S.P. Theor Appl Genet 86:81–87.

    CAS  Google Scholar 

  • Isabel N., Boivin R., Levasseur C., Charest P.-M, Bousquet J., Tremblay F.M. 1996 Occurrence of somaclonal variation among somatic embryo-derived white spruces(Picea glaucaPinaceae). Amer J Bot 83:1121–1130.

    Article  Google Scholar 

  • Ishii K., Moran G.F., Bell J.C., Hartney V. 1987 Genetic stability examination of micropropagated radiata pine(Pinus radiata)using isozyme assays. J.lap For Soc 69:487–488.

    Google Scholar 

  • Jain S.M., Ishii K., Bruns, S. 1998 Recent advances in somatic embryogenesis in forest trees. In: Proceedings of an IFS Workshop, Florianopolis, Brazil. 15–19 Sept. 1997. International Foundation os Sciences, Stockholm. pp. 214–231.

    Google Scholar 

  • Kantharajah A.S., Dodd W.A. 1990In vitromicropropagation ofPassiflore eduhs(purple passionfruit). Ann. Bot. 63:337–339.

    Google Scholar 

  • Karp A. 1994 Origins, causes and uses of variation in plant tissue cultures. In: Vasil, I.K. and T.A. Thorpe (eds). Plant Cell and Tissue Culture. Kluwer Academic, Dordrecht. pp. 139–151.

    Google Scholar 

  • Karp A.. Steele S.H.. Parmar S., Jones M.G.K., Shewry P.R. 1987 Relative stability among barley plants regenerated from cultured immature embryos. Genome 29:405–412

    Article  Google Scholar 

  • Kawata K., Ushida C., Kawai F., Kanamori M., Kuriyama A. 1995 Micropropagation of passionfruit from subcultured multiple shoot primordia. J Plant Physiol 147:281–284.

    Article  CAS  Google Scholar 

  • Khavkin E.F., Zabrodina M.V. 1994 Heritable variations in peroxidase and esterase isozyme patterns of maize somaclones. Russ J Plant Phys 41:859–867.

    CAS  Google Scholar 

  • Kumar V., Radha A., Kumar Chitta S. 1998In vitroplant regeneration of fig(Ficus car caL. cv. Gular) using apical buds from mature trees. Plant Cell Rep 17(9): 717–720.

    Article  CAS  Google Scholar 

  • Lardet L., Aguilar M.E., Michaux-Ferriere N., Berthouly M. 1998 Effect of strictly plant-related factors on the response ofHevea brasiliensisandTheobroma cacaonodal explants culturedin vitro. In VitroCell Dev Biol - Plant 34:34–40.

    Google Scholar 

  • Larkin P.J. 1987 Somaclonal variation: history, method, and meaning. Iowa St. J. Res., 61:393–434.

    Google Scholar 

  • Larkin P.J., Scowcroft W.R. 1981 Somaclonal variation-a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214.

    Article  Google Scholar 

  • Liew T.K., Teo C.K.H. 1998. Multiple shoot productionin vitroof the tropical timber tree, sentang(Azadirachta excelsaL.). HortSci 33:1073–1075.

    Google Scholar 

  • Lima M.M. de, Gonçalves A.N. 1998 Efeito do thidiazuron na multiplicaçáoin vitrode gemas de um clone deEucalyptus grandis x E. tereticornis.Sci Forestalis 53:49–56.

    Google Scholar 

  • Lin J.-J. Kuo J. 1995 AFLPTm: a novel PCR-based assay for plant and bacterial DNA fingerprinting. Focus 17:66–70.

    Google Scholar 

  • Liverani A., Cobianchi D., De Salvador F.R., Insero O., Minguzzi A. 1989 Field performance of two peach and nectarine cultivars propagatedin vitro.Acta Hort 254:41–46.

    Google Scholar 

  • Lloyd G., McCown B. 1980 Commercially-feasible micropropagation of Mountain LaurelKalmia latifoliaby use of shoot-tip culture. Comb. Proc. Intl. Plant Prop. Soc. 30:421427.

    Google Scholar 

  • Malavolta E., Vitti G.C., Oliveira S.A. 1997 XXXAvaliaçao do estado nutricional das plantas: principios e aplicaçóes. 2 ed. POTAFOS, Piracicaba. 319p.

    Google Scholar 

  • Manders G., Otoni W.C., D’utra Vaz F.B., Blackhall N.W., Power J.B., Davey M.R. 1994 Transformation of passionfruit(Passiflora edulisf.flavicarpaDegener.) usingAgrobacterium tumefaciens.Plant Cell Rep. 13:697–702.

    Article  CAS  Google Scholar 

  • Mendanha A.B.L., Torres R.A.D., Freire A.D. 1998 Micropropagation of rubber tree(Hevea brasiliensisMuell. Arg.). Genetics and Molecular Biol 21:395–398.

    Google Scholar 

  • Meyer H.J. 1998In vitroformation of adventitious buds on mature embryos ofPinus elliottiiEngelm. xP. caribaeaMorelet hybrids. South African J Bot 64:220–225.

    Google Scholar 

  • Monteiro A.C.B.A. 2000 Cultivoin vitrode trés espécies do gêneroPassiflora.MS thesis. ESALQ, University of Sao Paulo, Piracicaba. 82 p.

    Google Scholar 

  • Monteiro A.C.B.A., Higashi E.N., Gonçalves A.N., Rodriguez A.P.M. 2000A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis Sims. f. flavicarpa Deg.). In VitroCell Dev Biol - Plant 36:527–531.

    Article  Google Scholar 

  • Moran Robles M. J. 1978 Multiplication végétativein vitrodes bourgeons axillares dePassiflora edulisvar.flavicarpaDegener et deP. mollissimaBailey. Fruits 33:693–699.

    Google Scholar 

  • Moran Robles M. J. 1979 Potentiel morphogénétique des entrenoeuds dePassiflora edulisvar.flavicarpaDegener etP. mollissimaBailey en culturein vitro.Turrialba 29:224–228.

    Google Scholar 

  • Murashige T., Skoog F. 1962 A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiologia Plant 15:473–497.

    Article  CAS  Google Scholar 

  • Naik S.K., Pattnaik S., Chand P.K. 1999In vitropropagation of pomegranate(Punica granatumL. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature trees. Sci Hort 79:175–183.

    Article  CAS  Google Scholar 

  • Naik S.K., Pattnaik S., Chand P.K. 2000 High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate(Punica granatumL.). Sci Hort 85:261–270.

    Article  CAS  Google Scholar 

  • Nadwani D., Kumaria S., Tandon P. 2001 Micropropagation ofPinus kesiyaRoyle ex Gord (Khasi pine). Gartenbauwissenschaft 66:68–71.

    Google Scholar 

  • Nehra N.S., Kartha K.K., Stushnoff C., Giles K.L. 1992 The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry. Plant Cell Tiss Org Cult 29:257–268.

    Article  CAS  Google Scholar 

  • Normah M.N., Nor-Azza A.B., Aliudin R. 1995 Factors affectingin vitroshoot proliferation andex vitroestablishment of mangosteen. Plant Cell Tiss Org Cult 43:291–294.

    Google Scholar 

  • Otoni W.C., Casali V.W.D., Cecon P.R., Davey M.R., Power J.B. 1995 Regeneraçáo de plantas de maracujazeiro(Passiflora coccineaAubl.) a partir de protoplastos derivados de mesófilo. Rev Ceres 42:461–468.

    CAS  Google Scholar 

  • Pereira-Netto A.B. 1996In vitropropagation ofHancornia speciosaa tropical fruit tree.In VitroCell Dev Biol Plant 32:253–256.

    Article  Google Scholar 

  • Pereira-Netto A.B., McCown B.H. 1999 Thermally induced changes in shoot morphology ofHancornia speciosamicrocultures: evidence of mediation by ethylene. Tree Phys 19:733–740.

    Article  Google Scholar 

  • Perrin Y.. Doumas P.. Lardet L.. Carron M-P. 1996 Endogenous cytokinins as biochemical markers of rubbertree(Hevea brasiliensis)clone rejuvenation. Plant Cell Tiss Org Cult 47:239–245.

    Article  CAS  Google Scholar 

  • Quoirin M., Lepoivre P. 1977 Etude de milieux adaptes aux cultures in vitrodePrunus.Acta Hort 78:437–442.

    Google Scholar 

  • Rafalski J.A., Tingey S.V. 1993 Genetic diagnosis in plant breeding: RAPDs, microsatellites and machines. Trends in Genetics 9:275–280.

    Article  PubMed  CAS  Google Scholar 

  • Ragot M., Hoisington D.A. 1993 Molecular markers for plant breeding: comparisons of RFLP and RAPD genotyping costs. Theor Appl Genet 86:975–984.

    Article  CAS  Google Scholar 

  • Reuter D.J., Robinson J.B. (eds.) 1997 Plant analysis: an interpretation manual. 2 ed. CSIRO, Collingwood. 572p.

    Google Scholar 

  • Reuveni O., Israeli Y. 1990 Measures to reduce somaclonal variation inin vitropropagated bananas. Acta Hort 275:307–313.

    Google Scholar 

  • Richard S., Gauthier S.. Laliberté 5.1995 Isozymes assessment of the genetic stability of micropropagated hybrid larch(LarixXeurolepsisHenry). Can J For Res 25:1103–1112

    Article  CAS  Google Scholar 

  • Ritchie G.A., Long A.J. 1986 Field performance of micropropagated Douglas Fir. New Zeal J For Sci 16:343–356.

    Google Scholar 

  • Sabir A., Newbury H.J., Todd G., Catty J., Ford-Lloyd B.V. 1992 Determination of genetic stability using isozymes and RFLPs in beet plants regeneratedin vitro.Theor Appl Genet 84:113–117.

    Article  CAS  Google Scholar 

  • Smith R. 1992 Plant tissue culture: techniques and experiments. Academic Press, San Diego.

    Google Scholar 

  • Sposito G., Mattigod S.V. 1980 Geochem: a computer program for the calculation of chemical equilibria in soil solutions and other material water systems. Kearney Foundation of Soil Science, University of California, Riverside. 107p.

    Google Scholar 

  • Sujatha M., Chandran K.. 1997 A commercially feasible micropropagation method forMelia azedarachL. Indian Journ of Experimental Biology 35:787–791.

    Google Scholar 

  • Sunnichan V.G., Shivanna K.R., Ram H.Y.M. 1998 Micropropagation of gum karaya(Sterculia urens)by adventitious shoot formation and somatic embryogenesis. Plant Cell Rep 17:951–956.

    Article  CAS  Google Scholar 

  • Taylor P.W.J., Geijskes J.R., Ko H.-L., Fraser T.A., Henry R.J., Birch R.G. 1995 Sensitivity of random amplified polymorphic DNA analysis to detect genetic change in sugarcane during tissue culture. Theor Appl Genet 90:1169–1173.

    Article  CAS  Google Scholar 

  • Tetsumura T.. Yukinaga H. 1998 Early field performance of micropropagated Japanese persimmon trees. HortSci 33:751–753.

    Google Scholar 

  • Tibok A., Blackhall N.W., Power, J.B., Davey, M.R. 1995 Optimized plant regeneration from callus derived from seedling hypocotyls ofEucalyptus urophylla.Plant Science 110:139–145.

    Article  CAS  Google Scholar 

  • USDA, ARS, National Genetic Resources Program. 2001Germplasm Resources Information Network - (GRIN).[Online Database] National Germplasm Resources Laboratory, Beltsville, Maryland.

    Google Scholar 

  • Vallés M.P., Wang Z.Y., Montavon P., Potrykus l., Spangenberg G. 1993 Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue(Festuca pratensisHuds.). Plant Cell Rep 12:101–106.

    Article  Google Scholar 

  • Valverde-Cerdas L., Dufour, M., Villalobos V. 1998In vitroorganogenesis inAlbizia guachapele Cedrella odorataandSwietenia macrophylla(Fabaceae, Meliaceae). Rev. Biol Trop 46:225–228.

    Google Scholar 

  • Vaz R.L. 1991 A biotecnologia aplicada á fruticultura: necessidades e perspectivas potenciais. In. San José A.R., Ferreira, F.R., Vaz, R.L. (eds.). A cultura do maracujá no Brasil. FUNEP, Jaboticabal. p. 241–247.

    Google Scholar 

  • Vendrame W.A., Kochert G.D., Wetzstein H.Y. 1999 AFLP analysis of variation in pecan somatic embryos. Plant Cell Rep 18:853–857.

    Article  CAS  Google Scholar 

  • Vendrame W.A., Kochert G.D., Sparks D., Wetzstein H.Y. 2000 Field performance and molecular evaluations of pecan trees regenerated from somatic embryogenic cultures. J Amer Soc Hort Sci 125:542–546.

    CAS  Google Scholar 

  • Vuylsteke D., Swennen R., Wilson G.F., De Langhe E. 1988 Phenotypic variation among in-vitro propagated plantain(Musasp. cultivar AAB). Sci Hort 36:79–88.

    Article  Google Scholar 

  • Warner G. 1995 Identity query halts rootstock production. Good Fruit Grower, 46(16):1823.

    Google Scholar 

  • Warrag E.I., Lesney M.S. Rockwood D.L. 1989 Comparative greenhouse study ofEucalyptus grandis in vitroplantlets and half-sib seedlings. L Net photosynthesis. Plant Cell Rep., 8:497–499.

    Article  CAS  Google Scholar 

  • Warrag E.I.. Lesney M.S., Rockwood D.L. 1989 Comparative greenhouse study ofEucalyptus grandis in vitroplantlets and half-sib seedlings, II. Dry matter accumulation and relative distribution. Plant Cell Rep 8:500–503.

    Article  Google Scholar 

  • Webster A.D., Oehl V.H., Jackson J.E., Jones O.P. 1985 The orchard establishment, growth and precocity of four micropropagated apple scion cultivars. J Hort Sci 60:169–180

    Google Scholar 

  • Wetzstein H.Y., Myers S.C. 1994 Vegetative and yield component characteristics of micropropagated muscadine grape(Vitis rotundifoliaMichx.). J Hort Sei 69:747–753.

    Google Scholar 

  • Xavier. A., Comério, J. 1997 Enraizamentoex vitrode gemas deEucalyptusspp.multiplicadas e alongadas in vitro.Sci Forestalis 51:29–36.

    Google Scholar 

  • Zimmerman R.H. 1997 Orchard variation in micropropagated trees of ‘Redspur Delicious’ apple. HortSci 32:935–936.

    Google Scholar 

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Rodriguez, A.P.M., Vendrame, W.A. (2003). Micropropagation of Tropical Woody Species. In: Jain, S.M., Ishii, K. (eds) Micropropagation of Woody Trees and Fruits. Forestry Sciences, vol 75. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0125-0_6

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