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Micropropagation of Woody Trees and Fruits: Pathogen Elimination and Contamination Management

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Micropropagation of Woody Trees and Fruits

Part of the book series: Forestry Sciences ((FOSC,volume 75))

Abstract

Losses due to bacterial contamination of cultures and the risk of the release of pathogens in planting material produced by micropropagation are serious treats to the industry (Cassells, 2000a; Leifert and Cassells, 2001). In early conferences on contamination in tissue culture(e.g.Cassells, 1988), health status was discussed without reference to microplant quality, however, it was evident from the losses to pathogens at weaning (Williamsonet al., 1997) that microplant epigenetic and physiological quality are important in the process. It is now recognised that aseptic microplants are a ‘biological vacuum’ lacking the biological buffering of a natural microflora against opportunistic pathogens (Cassells, 2000b). This leads to efforts both to improve the physiological quality of microplantsin vitroand to biotization or inoculationin vitroandpost vitrumwith beneficial micro-organisms (Vestberget al.,2001). Both the health status and genetic, epigenetic, and physiological quality of microplants have been the subject of several conferences in recent years (Cassells 1997; Cassellset al.,2000). An emerging consensus on pathogen and contamination management and protection against damping-off diseases at weaning is that these may be best regarded as three separate phases in the micropropagation process.

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  1. Department of Plant Science, National University of Ireland Cork, Ireland

    Alan C. Cassells & Eileen A. O’Herlihy

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  1. Alan C. Cassells
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  1. FAO/IAEA Joint Division, International Atomic Agency, Vienna, Austria

    S. Mohan Jain

  2. Forestry and Forest Products Research Institute, Tsukuba, Japan

    Katsuaki Ishii

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Cassells, A.C., O’Herlihy, E.A. (2003). Micropropagation of Woody Trees and Fruits: Pathogen Elimination and Contamination Management. In: Jain, S.M., Ishii, K. (eds) Micropropagation of Woody Trees and Fruits. Forestry Sciences, vol 75. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0125-0_4

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  • DOI: https://doi.org/10.1007/978-94-010-0125-0_4

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