Skip to main content
SpringerLink
Log in

Micropropagation of Woody Plants Using Bioreactor

  • Chapter
Book cover Micropropagation of Woody Trees and Fruits

Part of the book series: Forestry Sciences ((FOSC,volume 75))

Abstract

The use of plant tissue culture techniques has revolutionized the plant propagation and now plants are commercially micropropagated. In recent years, many micropropagation companies world over have emerged. However, few companies are generating good profits. As a result micropropagation industry has not grown appreciably. High skilled labour cost in developed countries is the limiting factor for the development of this industry. Commercial laboratories so far, have been using the procedures developed at the research level in which skilled operators manipulate explants and sub-culture of shoots in laminer hood. The methods limit the total number of propagules a person can handle. This restricts its application to plants in which a high unit cost of production can be economically justified (Christie et al., 1995; Cervelli and Senaratna, 1995). In addition, current method of tissue culture is difficult to automate. Many significant biological and cultural problems must be solved before the technique can be amenable to automation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Akula A., Becker D., Bateson M. High yielding repetitive somatic embryogenesis and plant recovery in a selected tea clone, ‘TRI-2025’, by temporary immersion. Plant Cell Rep. 2000; 19:1140–1145.

    Article  CAS  Google Scholar 

  • Archambault J., Williams R. D., Lavoie L., Pepin M. F., Chavarie C. Production of somatic embryos in a helical ribbon impeller bioreactor. Biotechnol. Bioeng. 1994; 44: 930–943.

    Article  CAS  Google Scholar 

  • Attree S. M., Pomeroy M. K., Fowke L. C. Production of vigorous, desiccation tolerant white spruce (Picea glauca [Moench.] Voss.) synthetic seeds in a reactor. Plant Cell Rep. 1994; 13: 601–606.

    Article  Google Scholar 

  • Bapat V A., Fulzele D. P., Heble M. R., Rao P. S. Production of sandalwood somatic embryos in bioreactors. Curr. Sci 1990; 59: 746–748.

    Google Scholar 

  • Buitelaar R. N., Langenhoff A. A. N., Heidstra R., Tramper J. Growth and theophene production by hairy root cultures of Tagetes patula in various two liquid phase bioreactors. Enzyme Microbiol. Tech. 1991; 13: 487–494.

    Article  CAS  Google Scholar 

  • Cabasson C., Alvard D., Dambeir D., 011itrault P., Teisson C. Improvement of Citrus somatic embryo development by temporary immersion. Plant Cell Tiss. Org. Cult. 1997; 50:33–37.

    Google Scholar 

  • Cervelli R., Senaratna T. Economic aspects of somatic embryogenesis. In: Automation and environmental control in plant tissue culture. Christie J. A., Kozai T., Smith M. L., eds. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Christie J. A., Kozai T., Takayama S. Automation in plant tissue culture-general introduction and overview. In: Automation and environmental control in plant tissue culture. Christie J. A., Kozai T., Smith M. L., eds. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Damiano C., Caboni E., Frattarelii A., Giorgioni M., Liberali M., Lauri P., Angeli S. D. Innovative micropropagation of temperate fruit trees: the case of pear. Acta Hortic. 2000; 530: 181–185.

    Google Scholar 

  • Das S., Das S., Pal S., Ponde N. R., Gupta S. D., Mujib A., Sahoo S. S., Dey S. A novel process for rapid mass propagation of Santalum album L. in liquid media and bioreactor. Acta Hortic. 1999; 502: 281–288.

    Google Scholar 

  • Denchev P. D., Kuklin A. I., Scragg A. H. Somatic embryo production in bioreactors. J. Biotech. 1992; 26: 99–109.

    Article  CAS  Google Scholar 

  • Dunstan D. I., Tautorus T. E., Thorpe T. A. Somatic embryogenesis in woody plants. In: In vitro embryogenesis in plants. Thorpe T. E., ed. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Escalona M., Lorenzo J. C., Gonzalez B., Daquïnta M., Gonzalez J. L., Desjardins, Y., Borroto, C. G Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems. Plant Cell Rep. 1999; 18:743–748.

    Article  CAS  Google Scholar 

  • Etienne D. B., Bertrand B., Schlonvoigt A., Ettienne H. The morphological variability within a population of coffee somatic embryos produced in a bioreactor affects the regeneration and the development of plants in the nursery. Plant Cell Tiss. Org. Cult. 2002; 68: 153–162.

    Google Scholar 

  • Etienne D. B., Bertrand B., Vasquez N., Etienne H. Direct sowing of Coffea arabica somatic embryos mass produced in a bioreactor and regeneration of plants. Plant Cell Rep. 1999; 19:111–117.

    Article  Google Scholar 

  • P. H., Olsen O. A. S., Eide A. K. H. Engineering aspects of plant propagation in bioreactors. In: Automation and environmental control in plant tissue culture. Christie J. A., Kozai T., Smith M. L., eds. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Honda H., Liu C., Kobayashi T. “Large scale Plant propagation”. In Advances in Biochemical Engineering/Biotechnology, Vol. 72, Scheper T., ed. Springer-Verlag Berlin Heidelberg, 2001.

    Google Scholar 

  • Ibaraki Y, Kurata K. Automation of somatic embryo production. Plant Cell Tiss. Org. Cult. 2001: 65: 179–199.

    CAS  Google Scholar 

  • Ingram B., Mavituna F. Effect of bioreactor configuration on the growth and maturation of Picea sitchensis somatic embryo cultures. Plant Cell Tiss. Org. Cult. 2000; 61: 87–96.

    Google Scholar 

  • Ishibashi T., Odawara Y, Fujiwara K., Ookuma M. New oxygen supplying method for animal cell reactors. Hitachi Hyouron. 1987; 69: 301–305 (in Japanese).

    Google Scholar 

  • Jain S. M., Gupta P. K. and Newton R. J (Eds.). Somatic embryogenesis in woody plant. Volumes 1–3, 1995; Kluwer Academic Publishers, The Netherlands.

    Google Scholar 

  • Jain S. M., Gupta P. K. and Newton R. J (Eds.). Somatic embryogenesis in woody plants, Volumes 4–5, 1999; Kluwer Academic Publishers, The Netherlands.

    Google Scholar 

  • Jain S. M., Gupta P. K. and Newton R. J (Eds.). Somatic embryogenesis in woody plants, Volume 6, 2000; Kluwer Academic Publishers, The Netherlands.

    Google Scholar 

  • Kim J. W., Kim H. S. Mass production of Siberian ginseng (Eleutherococcus senticosus) somatic embryos by cell culturing. Oriental Pharmacy Exp. Med. 2001; 1:34–38.

    Google Scholar 

  • Kim Y. S. Development of large scale process for mass production of lily bulblets by bioreactor system. 1999; M. Sc thesis. Chungbuk National University, Korea.

    Google Scholar 

  • Konar R. N., Oberoi Y. P., In vitro development of embryoids on the cotyledons of Biota orientalis. Phytomorphology. 1965; 15: 137.

    CAS  Google Scholar 

  • Kurata K. Image analysis for embryogenesis. In: Automation and environmental control in plant tissue culture. Christie J. A., Kozai T., Smith M. L., eds. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Lee Y. H. Design of bioreactor system for small and pilot scale cultivation of plant cells. 1997; M. Sc. thesis. Chungbuk National University, Korea.

    Google Scholar 

  • Lian M. L. Several Factors affecting bulblet production in vitro and establishment of mass production system of Lilium Hybrid using bioreactor. 2001; Ph. D thesis. Chungbuk National University, Korea.

    Google Scholar 

  • Mavituna F., Buyukalaca S. Large-scale production of pepper somatic embryos using bioreactors. Acta Hortic, 1995; 412: 58–63.

    Google Scholar 

  • Mavituna F., Buyukalaca S. Somatic embryogenesis of pepper in bioreactors: a study of bioreactor type and oxygen uptake rates. Applied Micro. Biotech. 1996; 46: 327–333.

    CAS  Google Scholar 

  • Moorhouse S. D., Wilson G, Hennerty M. J., Selby C., Antsaoir S. M. A plant cell bioreactor with medium perfusion for control of somatic embryogenesis in liquid cell suspensions.Plant Growth Regul. 1996; 20: 53–56.

    Article  CAS  Google Scholar 

  • Nishihira T., Hayashi Y., Matsumoto K. Somatic embryo induction from cell suspension culture of Aralia cordata using bioreactors. J. Jap. Soc. Hort. Sci. 1998; 67: 87–92.

    Article  Google Scholar 

  • Paek, K. Y, Hahn E. J., Son S. H. Application of bioreactors for large-scale micropropagation systems of plants. In vitro Cell. Dev. Biol.-Plant. 2001; 37: 149–157.

    CAS  Google Scholar 

  • Paques M., Bercetche J., Palada M. Prospects and limits of somatic embryogenesis of Picea abies. In: Somatic embryogenesis in Woody Plants. Vol. 1. Jaín S., Gupta P, Newton R. eds. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Park S. Y, Murthy H. N., Paek K. Y Mass multiplication of protocorm like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tiss. Org. Cult. 2000; 63: 67–72.

    Google Scholar 

  • Preil W. Application of bioreactors in plant propagation. In: Micropropagation. Debergh P. C., Zimmerman R. H. eds. Kluwe Academic Publishers. 1991.

    Google Scholar 

  • Ray P. M., Steeves T. A., Fultz S. A. Botany. Saunders College Publishing, New York, 1983.

    Google Scholar 

  • Redenbaugh K., Fujii J., Slade D., Viss P., Kissler M. Artificial seeds-encapsulated somatic embryos. In: Biotechnology in Agriculture and Forestry. Y.P.S. Bajaj (Ed.) 1991; 17: 395–416.

    Article  Google Scholar 

  • Sajc L., Grubisic D., Novakovic G. V. Bioreactors for plant engineering: an outlook for further research. Biochem. Eng. J. 2000; 4: 89–99.

    Article  Google Scholar 

  • Sharma N. K. Automation in plant tissue culture: problems and prospects. Curr. Sci. 1992; 7:507–512.

    Google Scholar 

  • Shim S. W. Environmental conditions affecting in vitro growth and acclimatization of ‘SBB’ grape rootstock. 2002; Ph. D thesis. Chungbuk National University, Korea

    Google Scholar 

  • Son S. H., Choi S. M., Kwon S. R., Lee Y. H., Paek K. Y. Large Scale culture of plant cell and tissue by bioreactor system. J. Plant Biotech. 1999; 1: 1–8.

    Google Scholar 

  • Steward F. C., Mapes M. O., Mears K. Growth and organized development of cultured cells. II.Organization in cultures grown from freely suspended cells. Am. J. Bot. 1958; 45: 705.

    Article  Google Scholar 

  • Stuart D. A., Strickland S. G, Walker K. A. Bioreactor production of alfalfa somatic embryos. HortScience. 1987; 22: 800–803.

    Google Scholar 

  • Takayama S., Akita M. The types of bioreactors used for shoots and embryos. Plant Cell Tiss. Org. Cult. 1994; 39: 147–156.

    Google Scholar 

  • Takayama S., Misawa M. Mass propagation of Begonia hiemalis plantlets by shake culture. Plant Cell Physiol. 1981; 22: 461–467.

    CAS  Google Scholar 

  • Takayama S. Mass propagation of plants through shake and bioreactor culture techniques. In: Y.P.S. Bajaj (Ed.). Biotechnology in Agriculture and Forestry, Vol. 17 (pp 495–515). Springer-Verlag, Berlin.

    Google Scholar 

  • Tautorus T. E., Dunstan D. I.Scale-up of embryogenic plant suspension cultures in bioreactors. In: Jam S. M., Gupta P. K and Newton R. J (Eds.). Somatic embryogenesis in woody plant. Volumes 1–3, 1995; Kluwer Academic Publishers, The Netherlands.

    Google Scholar 

  • Tautorus T. E., Lulsdorf M. M., Kikcio S. I., Dunstan D. I. Bioreactor culture of Picea mariana Mill. (Black spruce) and the species complex Picea glauca-engelmannii (interior spruce) somatic embryos. App. Microbiol. Biotechnol. 1992; 38: 46–51.

    CAS  Google Scholar 

  • Tautorus T. E., Lulsdorf M. M., Kikcio S. I., Dunstan D. I. Nutrient utilization during bioreactor culture, and regeneration of somatic embryo cultures of Picea mariana and Picea glaucaengelmannii. In vitro Cell Dev. Biol. 1994; 30P: 58–63.

    CAS  Google Scholar 

  • Teisson C., Alvard D. A new concept of plant in vitro cultivation liquid medium: temporary immersion. In: Current issues in plant molecular and cellular biology. Terzi M., Cella R., FalavignaA. eds. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Timmis R. Bioprocessing for tree production in the forest industry: conifer somatic embryogenesis. Biotech. Progress. 1998; 14: 156–166.

    CAS  Google Scholar 

  • Walker P. N. System analysis and engineering. In: Automation and environmental control in plant tissue culture. Christie J. A., Kozai T., Smith M. L., eds. Kluwer Academic Publishers, Dordrecht. 1995.

    Google Scholar 

  • Yu K. W. Production of the useful metabolites through bioreactor culture of Korean ginseng(Panax ginseng C. A. Meyer). 2000; Ph. D thesis. Chungbuk National University, Korea

    Google Scholar 

  • Ziv M., Ariel T. Bud proliferation and plant regeneration in liquid cultured Philodendron treated with ancymidol and paclobutrazol. J. Plant Growth Reg. 1991; 10: 53–57.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, South Korea

    Paek Kee Yoeup & Debasis Chakrabarty

Authors
  1. Paek Kee Yoeup
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Debasis Chakrabarty
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. FAO/IAEA Joint Division, International Atomic Agency, Vienna, Austria

    S. Mohan Jain

  2. Forestry and Forest Products Research Institute, Tsukuba, Japan

    Katsuaki Ishii

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Yoeup, P.K., Chakrabarty, D. (2003). Micropropagation of Woody Plants Using Bioreactor. In: Jain, S.M., Ishii, K. (eds) Micropropagation of Woody Trees and Fruits. Forestry Sciences, vol 75. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0125-0_25

Download citation

  • DOI: https://doi.org/10.1007/978-94-010-0125-0_25

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3964-2

  • Online ISBN: 978-94-010-0125-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation