XYZ on a Chip: Nanoscale fabrication, fluidics, and optics directed toward applications within biology and medicine
We have developed techniques for fabrication of micro-scale and nano-scale structures in polymeric media and we have developed methodology for control of fluid flow within these systems. We have also developed methods for modeling fluid flow in these systems under both electrokinetically driven and hydrostatically driven conditions. These capabilities offer new opportunities for exploration of biological function in many systems. We have illustrated these capabilities for in vitro studies by examination of the influence of medium composition and flow rate on the growth of cells by studying cell volume and shape changes of chondrocyte cells and ACL fibroblast cells at regulated osmotic loadings close to physiological frequencies. We have also demonstrated the development of an artificial medium for study of the kinetics and biological function of biological cells with specific application to understanding the function of osteocyte and osteoblast cells within the bone structures. These experiments demonstrate the efficacy of new generations of bio-chips for studies of biological function.
KeywordsFluid Environment Electroosmotic Flow Dimensionless Frequency Outlet Channel Chondrocyte Cell
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