XYZ on a Chip: Nanoscale fabrication, fluidics, and optics directed toward applications within biology and medicine
We have developed techniques for fabrication of micro-scale and nano-scale structures in polymeric media and we have developed methodology for control of fluid flow within these systems. We have also developed methods for modeling fluid flow in these systems under both electrokinetically driven and hydrostatically driven conditions. These capabilities offer new opportunities for exploration of biological function in many systems. We have illustrated these capabilities for in vitro studies by examination of the influence of medium composition and flow rate on the growth of cells by studying cell volume and shape changes of chondrocyte cells and ACL fibroblast cells at regulated osmotic loadings close to physiological frequencies. We have also demonstrated the development of an artificial medium for study of the kinetics and biological function of biological cells with specific application to understanding the function of osteocyte and osteoblast cells within the bone structures. These experiments demonstrate the efficacy of new generations of bio-chips for studies of biological function.
KeywordsHydrolysis Sucrose Convection Platinum Electrophoresis
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- 4.Hong, S., Tang, Z., Djukic, D., Tucay, A., Bakhru, S., Osgood, R., Yardley, J., West, A.C., and Modi, V. (2001) Simulation and experimental validation of electroosmotic flow in a microfluidic channel 2001 Microelectromechanical Systems Conference, California, 73–76.Google Scholar
- 5.Tang, Z., Hong, S., Djukic, D., Modi, V., West, A.C., Yardley, James T., and Osgood, R. (in press) Electrokinetic Flow Control for Composition Modulation in a Microchannel Journal of Micromechanics and Microengineering.Google Scholar
- 8.Bird, Byron R., Stewart, Warren E., and Lightfoot, Edwin N. (1960) Transport Phenomena. Wiley, New York.Google Scholar
- 9.Chao, Grace, Tang, Zhongliang, Angelini, Elsa, West, Alan, and Hung, Clark (Accepted) A novel microfluidic device to study the real-time response of cultured cells to applied dynamic osmotic loading 49th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, Feb 2003.Google Scholar