Abstract
Bacteriocins are bactericidal proteins that are produced by bacteria. They usually kill only bacterial cells that are closely related to the producing bacteria. The narrow host range is determined by the sophisticated uptake mechanisms that these proteins use to enter susceptible bacterial cells. Bacteriocins range in size from rather small toxic peptides to relatively large protein structures of 60–70 kDa. The relatively small bactericidal peptides include microcins and lantibiotics (De Vos et al., 1995), most of which are apparently exported by the producing cell via ABC transporters. This chapter will focus on the larger bacteriocins, especially on colicins and cloacin DF13, and on their mechanism of export. These bacteriocins are the best studied bacteriocins with respect to production, export, uptake and mode of action. These relatively large proteins are produced by Escherichia coli strains and by related gram-negative organisms. The mechanism by which these colicins are translo cated across both the inner membrane and the outer membrane to reach their extra-cellular destination is totally different from all other mechanisms studied so far. This process appears to be semi-specific and is often referred to as quasi-lysis. The exact mechanism is not known, but it has been applied for the production of heterologous proteins by E. coli. In this chapter, the mechanism of export of colicins and of cloacin DF13, and the biotechnological applications of this mechanism of export will be described.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
8. References
Aono, R. (1988) Appl Microbiol Biotechnol 28: 414–418.
Baty, D., Lloubès R., Géli, V., Lazdunski, C. and Howard, S.P. (1987) EMBO J 6: 2463–2468.
Cavard, D., Crozel, V., Gorvel, J.P., Partus, F., Baty, D. and Lazdunski, C. (1986) J Mol Biol 187: 449–459.
Cavard, D., Baty, D., Howard, S.P., Verhey, H.M. and Lazdunski, C. (1987) J Bacteriol 169: 2187–2194.
Cavard, D., Howard, S.P. and Lazdunski, C. (1989) J Gen Microbiol 135: 1715–1726.
Cavard, D. (1991) J Bacteriol 173: 191–196.
Cullis, P.R. and De Kruijff, B. (1979) Biochim Biophys Acta 559: 399–420.
De Graaf, F. K. and Oudega, B. (1986) Curr Top Microbiol Immunol 125: 183–205.
Dekker, N., Tommassen, J. and Verhey, H.M. (1999) J Bacteriol 181: 3281–3283.
De Vos, W.M., Kuipers, O.P., Van der Meer, J.R. and Siezen, R.J. (1995.) Mol Microbiol 17: 427–437.
Elkins, P., Bunker, A., Cramer, W.A. and Stauffacher, V.V. (1997) Structure 5: 443–458.
Géli, V., Baty, D., Knibiehler, M., Lloubés, R., Pessegue, B., Shire, D. and Lazdunski, C. (1989) Gene 80: 129–136.
Gross, P. and Braun V. (1996) Mol Gen Genet 251: 388–396.
Horrevoets, A.J.G., Francke, C, Verheij, H.M. and De Haas, G.H. (1991) Eur J Biochem 198: 255–261.
Howard, S.P., Leduc, M., van Heijenoort, J. and Lazdunski, C. (1987) FEMS Microbiol Lett 42: 147–151.
Howard, S.P., Cavard, D. and Lazdunski, C. (1991) J Gen Microbiol 137: 81–89.
Howard, S.P. and Lindsay, L. (1992) In: Bacteriocins, microcins and lantibiotics. (James R., Lazdunski C, Pattus F.). Springer-Verlag, Berlin 317–329.
Hsiung, H.M., Mayne, N.G. and Becker, G.W. (1986) Bio/Technol 4: 991–995.
Hsiung, H.M., Cantrell,, A., Luirink, J., Oudega, B., Veros, A.J. and Becker, G.W. (1989) Bio/Technol 7: 267–271.
Hussain, M., Ozawa, Y., Ichihara, S. and Mizushima, S. (1982) Eur J Biochem 129: 223–239.
Kanoh, S., Masaki, H., Yajima, S., Ohta, T. and Uozumi, T. (1991) Agric Biol Chem 55: 1607–1614.
Kleanthous, C., Hemmings, A.M., Moore, G.R. and James, R. (1998) Mol Microbiol 28: 227–233.
Konisky, J. (1982) Ann Rev Microbiol 36: 125–144.
Lazdunski, C.J., Bouveret, E., Rigal, A., Journet, L., Lloubes, R. and Benedetti, H. (1998) J Bacteriol 180: 4993–5002.
Lloubès, R., Vita, N., Bernadac, A., Shie D., Leplatois, P., Géli V., Frenette, M., Knibiehler M., Lazdunski, C. and Baty, D. (1993) Biochimie 75: 451–458.
Luirink, J., van der Sande, C, Tommassen, J., Veitkamp, E., de Graaf, F.K. and Oudega, B. (1986) J Gen Microbiol 132: 825–834.
Luirink, J., de Graaf F.K. and Oudega, B. (1987) Mol Gen Genet 206: 126–132.
Luirink, J., Hayashi, S., Wu, H.C., Kater, M.M., de Graaf, F.K. and Oudega, B. (1988) J Bacteriol 170: 4153–4160.
Luirink, J., Watanabe, T., Wu, H.C., Stegehuis, F., de Graaf, F.K. and Oudega, B. (1987) J Bacteriol 169: 2245–2250.
Luirink, J. (1989) Thesis, Amsterdam.
Luirink, J., Duim, B., de Gier, J.W.L. and Oudega, B. (1991) Mol Microbiol 5: 393–399.
Oudega, B., Stegehuis, F., van Tiel-Menkveld G.J. and de Graaf, F.K. (1982) J Bacteriol 150: 1115–1121.
Parker, M.W., Postma, J.P., Pattus, F., Tucker, A.D. and Tsernoglou D. (1992) J Mol Biol 224: 639–657.
Pugsley, A.P. and Schwarz, M. (1983) J Bacteriol 156: 109–114.
Pugsley A.P. and Schwarz, M. (1983) Mol Gen Genet 190: 366–372.
Pugsley, A.P. (1984) Micro Sci 1: 168–175.
Pugsley, A.P. and Schwartz, M. (1984) EMBO J 3: 2393–2397.
Pugsley, A.P. and Cole, S.T. (1986) J Gen Microbiol 132: 2297–2307.
Pugsley, A.P. and Cole, S.T. (1987) J Gen Microbiol 133: 2411–2420.
Schoner, R.G., Ellis, L.F. and Schoner, B.E. (1985) Bio/Technol 2:151–154.
Steidler, L., Fiers, W. and Remaut, E. (1984) Biotechnol Bioeng 44: 1074–1082.
Snijder, H.J., Ubarretxena-Belandia, I., Blaauw, M., Kalk, K.H., Verhey, H.M., Egmond, M.R., Dekker N. and Dijkstra, B.W. (1999) Nature 401: 717–721.
Suit, J.L. and Luria, S.E. (1988) J Bacteriol 170: 4963–4966.
Wiener, M., Freymann, D., Ghosh, P. and Strouid R.M. (1997) Nature 385: 461–464.
Van der Wal F.J., Oudega B., Kater, M.M., ten Hagen-Jongman, C.M., de Graaf, F.K. and Luirink, J. (1992) Mol Microbiol 6: 2309–2318.
Van der Wal, F.J., Valent, Q.A., ten Hagen-Jongman, C.M., de Graaf, F.K., Oudega, B. and Luirink, J. (1994) Microbiology 140: 369–378.
Van der Wal F.J., Luirink J. and Oudega, B. (1995) FEMS Microbiol Rev 17: 381–399.
Van der Wal, F.J., Koningstein, G., ten Hagen, C.M., Oudega B. and Luirink J. (1998) Appl Env Microb 64: 392–398.
Van Tiel-Menkveld, G.J., Veitkamp, E. and de Graaf, F.K. (1981) J Bacteriol 146: 41–48.
Wu, H.C. and Tokunaga, M. (1986) Curr Top Microbiol Immunol 125:127–157.
Yu, P. and San, K.Y. (1992) Biotech Progress 8: 25–29.
Yu, P. and San, K.Y. (1993) Biotech Progress 9: 587–593.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Oudega, B. (2003). Export of Bacteriocins. In: Oudega, B. (eds) Protein Secretion Pathways in Bacteria. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0095-6_12
Download citation
DOI: https://doi.org/10.1007/978-94-010-0095-6_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3974-1
Online ISBN: 978-94-010-0095-6
eBook Packages: Springer Book Archive