Abstract
Bacteria appear to be simply organized organisms, but bacterial cells do have different subcellular compartments: the cytoplasm, the cytoplasmic or inner membrane (IM), the cell wall and in the case of gram-negative bacteria, the periplasm and the outer membrane (OM). Proteins are synthesized in the cytosol, and a large number of these proteins fold in the cytoplasm and play their role in this compartment. However, another large number of proteins (about 40 % of the total amount of proteins) is destined for functioning in one of the extra-cytoplasmic environments. These proteins have to be targeted to the IM, during or after completion of translation, and they have to be inserted into or translocated across this membrane. The mechanisms by which extra-cytoplasmic proteins are inserted into or translocated across the IM, transported to their final destination, fold and function, are described in more detail in one of the following chapters of this book. This chapter will deal with the first steps in these translocation pathways: the early recognition of membrane proteins and of secreted proteins by various targeting factors, and their targeting to generic or specific protein translocation sites (translocons) in the IM of Escherichia coli.
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Luirink, J., Oudega, B. (2003). Protein Targeting to the Inner Membrane. In: Oudega, B. (eds) Protein Secretion Pathways in Bacteria. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0095-6_1
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