Application of recA PCR-RFLP for Differentiation of Erwinia carotovora subsp. carotovora and Erwinia carotovora subsp. atroseptica.

  • M. Waleron
  • K. Waleron
  • E. Lojkowska


The species Erwinia carotovora comprises pectolytic erwinias that cause diseases on wide range of plants. Erwinia carotovora subsp. atroseptica (Eca) causes blackleg and soft rot of potato and in some countries is considered a quarantine pathogen. Erwinia carotovora subsp. carotovora (Ecc) has much wider host ranges and is considered an ubiquitous pathogen. The mixture of bacteria from both subspecies can be isolated from rotting tissue of potato plants and tubers, the ratio depending on infection pressure and environmental conditions (7). Our work indicated that about 57 % of Erwinia isolates detected in potato plants and tubers in Poland were identified as Eca (9).


Potato Plant Wide Host Range Infection Pressure Erwinia Carotovora International Collection 
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  1. 1.
    Ausubel, F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J.A., and Struhl K. 1992. Short protocols in molecular biology. Second edition.Google Scholar
  2. 2.
    Darrasse, A., Priou S., Kotoujansky A., Bertheau Y. 1994. PCR and Restriction Fragment Lenght Polymorphism of a pel gene as a tool to identify Erwinia carotovora in relation to potato diseases. Appl. Environ. Microbiol. 60: 1437–1443,PubMedPubMedCentralGoogle Scholar
  3. 3.
    De Boer, S. H., and Ward L. J. 1995. PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue. Phytopathology 85:854–858.CrossRefGoogle Scholar
  4. 4.
    Fréchon D., Exbrayat P., Helias V., Hyman L.J., Jouan B., Llop P. Lopez M.M., Payet N., Perombelon M.C.M., Toth I.K., Van Beckhoven J.R.C.M., Van der Wolf J.M. and Bertheau Y. 1998. Evaluation of a PCR kit for the detection of Erwinia carotovora subsp. atroseptica on potato tubers. Potato Res. 41: 163–173.CrossRefGoogle Scholar
  5. 5.
    Maki-Valkama, T. and Karjalainen R. 1994. Differentiation of Erwinia carotovora subsp. atroseptica and carotovora by RAPD-PCR. Annals of Appl. Biol. 125, 301–309.Google Scholar
  6. 6.
    Parent J.G., Lacroix M., Page D., and Vezinz L. 1996. Identification of Erwinia carotovora from soft rot plants by random amplified polymorphic DNA (RAPD) analysis. Plant Dis. 80: 494–460.CrossRefGoogle Scholar
  7. 7.
    Perombelon, M.C.M., and Hyman, L.J. 1987. Frequency of Erwinia carotovora in the Alyth Burn in eastern Scotland and the sources of the bacterium. J. Appl. Bacteriol 63:281–291.CrossRefGoogle Scholar
  8. 8.
    Smid E.J., Jansen A.H.J, and Gorris L.G.M. 1995. Detection Erwinia carotovora subsp. atroseptica and Erwinia carotovora subsp. carotovora in potato tubers using polymerase chain reaction. Plant Dis. 74: 505–509.Google Scholar
  9. 9.
    Sledz W., Jafra S., Waleron M. and Łojkowska E. 2000. Genetic diversity of Erwinia carotovora strains isolated from infected plants growing in Poland. EPPOBulletin (in press).Google Scholar
  10. 10.
    Ward L.J. and De Boer S.H. 1990. A DNA probe specific for serologically diverse strains of Erwinia carotovora. Phytopathology 80:665–669.CrossRefGoogle Scholar
  11. 11.
    Zhao X., McEntee K. 1990. DNA sequence analysis of the recA genes from Proteus vulgaris, Erwinia carotovora, Shigella flexneri and Escherichia coli B/r. Mol. Gen. Genet. 222:369–376.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • M. Waleron
    • 1
  • K. Waleron
    • 1
  • E. Lojkowska
    • 1
  1. 1.Department of Biotechnology, Faculty of Biotechnology UG & AMGLaboratory of Plant Protection & BiotechnologyGdanskPoland

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