Abstract
Infections of wound sites in dicotyledonous plants by Agrobacterium tumefaciens lead to the formation of crown gall tumors. The mechanism of pathogenesis involves the transfer of a segment of DNA (the T DNA) from a bacterial plasmid to the host cell. Bacterial attachment to host cells is required for virulence. All nonattaching bacterial mutants presently described are avirulent(2). Previously we have described the isolation of 3 transposon mutants of A. tumefaciens strain C58 which are unable to attach to plant cells and are avirulent (1). These mutants were isolated by screening random Tn5 mutants for failure to attach to carrot suspension culture cells. This report describes the characterization of the region of the bacterial chromosome in which these the nonattaching mutants are located.
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References
Matthysse, A. G. 1987. Characterization of nonattaching mutants of Agrobacterium tumefaciens. J. Bacterid. 169:313–323.
Matthysse, A. G., H. A. Yarnall, S. B. Boles, and S. McMahan. 2000. A region of the Agrobacterium tumefaciens chromosome containing genes required for virulence and attachment to host cells. Biochim. Biophys. Acta 1490:208–212.
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© 2001 Springer Science+Business Media Dordrecht
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Matthysse, A.G., Yarnall, H., Boles, S.B., McMahan, S. (2001). Agrobacterium tumefaciens Chromosomal Genes Required for Virulence and Attachment to Host Cells. In: De Boer, S.H. (eds) Plant Pathogenic Bacteria. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0003-1_40
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DOI: https://doi.org/10.1007/978-94-010-0003-1_40
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3858-4
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