Abstract
Fire blight is a necrotic disease, which affects fruit trees like apple and pear and some ornamentals (e.g. Cotoneaster and Crataegus spp.) (6). Most European countries and the Eastern Mediterranean area are affected (7). The spreading of the causative bacterium E. amylovora can occur over short distances for example via insects but also over longer distances via birds, wind and asymptomatic plant propagation materials. Recent outbreaks of the disease in Italy (9) and Spain (4) renewed the question about the origin of the pathogen. To describe the epidemic spread of fire blight we have applied two molecular methods: Pulsed Field Gel Electrophoresis (PFGE) (8,9) and determination of short sequence DNA repeats (SSR analysis) (2,3,5).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Bereswill, S., Pahl, A., Bellemann, P., Zeller, W., and Geider, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by Polymerase Chain Reaction-analysis. Applied and Environmental Microbiology 58: 3522–3526.
Kim W. -S., and Geider K. 1999. Analysis of variable short-sequence DNA repeats on the 29-kb plasmid of Erwinia amylovora strains. European Journal of Plant Pathology 105: 703–713.
Lecomte, P., Manceau, C., Paulin, J.P, and Keck M. 1997. Identification by PCR analysis on plasmid pEA29 of isolates of Erwinia amylovora responsible of an outbreak in Central Europe. European Journal of Plant Pathology 103: 91–98.
Lopez, M. M., Gorris, M. T.; Llop, P., Cambra, M., Rosello, M., Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, J.L. 1999. Fire blight in Spain: situation and monitoring. Acta Horticulturae 489: 187–191.
Schnabel E.L., and Jones A.L. 1998. Instability of a pEA29 marker in Erwinia amylovora previously used for strain classification. Plant Disease 82: 1334–1336.
Van der Zwet T., and Keil H.L. 1979. Fire blight: A bacterial disease of rosaceous plants. Agriculture Handbook no. 510, US Government Printing Office, Washington. 200 pp.
Van der Zwet T. 1996. Present worldwide distribution of fire blight. Acta Horticulturae 411: 7–8.
Zhang, Y., and Geider K. 1997. Differentiation of Erwinia amylovora strains by pulsed field gel electrophoresis. Applied and Environmental Microbiology 63: 4421–4426.
Zhang, Y., Merighi, M., Bazzi, C., and Geider K. 1998. Genomic analysis by pulsed-field gel electrophoresis of Erwinia amylovora strains from the Mediterranean region including Italy. Journal of Plant Pathology 80: 225–232.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Jock, S., Kim, WS., Bazzi, C., Lopez, M.M., Geider, K. (2001). Molecular Differentiation of Erwinia amylovora Strains from Europe and the Mediterranean Region. In: De Boer, S.H. (eds) Plant Pathogenic Bacteria. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0003-1_23
Download citation
DOI: https://doi.org/10.1007/978-94-010-0003-1_23
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3858-4
Online ISBN: 978-94-010-0003-1
eBook Packages: Springer Book Archive