Co-Operative Conformational Properties of Nucleosides, Nucleotides and Nucleotidyl Units in Solution

  • David B. Davies
Conference paper
Part of the The Jerusalem Symposia on Quantum Chemistry and Biochemistry book series (JSQC, volume 11)

Abstract

Nuclear magnetic resonance spectroscopy has made an important contribution to an understanding of the conformations and interactions of nucleic acids derivatives in solution (Davies 1978a). Methods are available for determining detailed descriptions of sugar ring conformations (Altona and Sundaralingam 1972, 1973) and the conformational properties of four out of the six bonds of the sugar-phosphate backbone utilising 1H-1H, 1H-3 1P or 1 3C-3 1P vicinal coupling constants (Davies, 1978a). Many nmr methods have been used to determine glycosidic bond conformations in a qualitative manner. No method provides an unequivocal description of the syn and anti conformational ranges or the equilibrium between them but methods based on relaxation measurements {n.O.e (Guéron et al, 1973) and T1 measurements (Lüdemann et al, 1975; Chachaty et al, 1976)} and 3 J(13C, H1′) observations (Lemieux, 1973; Davies, 1976) offer the possibility of quantitative determinations of glycosidic bond conformations. Each single bond is taken to be flexible and appropriate nmr parameters are analysed in terms of the time-averaged equilibrium of contributions from a number of conformers for each bond. The available conformational regions are defined, as far as possible, by those observed in the solid state by X-ray crystallography (Sundaralingam, 1973; Jack et al, 1976).

Keywords

Sugar Oligomer Pyrimidine Purine Nucleoside 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Altona, C.: 1975, Structure and Conformations of Nucleic Acids and Protein-Nucleic Acid Interations (Sundaralingam, M. and Rao, S. T. eds.), Univ. Park Press, Baltimore, pp. 613–629Google Scholar
  2. Altona, C. and Sundaralingam, M.: 1972, J. Amer. Chem. Soc., 94, 8205CrossRefGoogle Scholar
  3. Altona, C. and Sundaralingam, M.: 1973, J. Amer. Chem. Soc., 95, 2333CrossRefGoogle Scholar
  4. Chachaty, C., Zemb, T., Langlet, G. and Tran-Dinh, S.: 1976, Eur. J. Biochem., 62, 45CrossRefGoogle Scholar
  5. Davies, D. B. and Danyluk, S. S.: 1974, Biochemistry, 13, 4417CrossRefGoogle Scholar
  6. Davies, D. B. and Danyluk, S. S.: 1975, Biochemistry, 14, 543CrossRefGoogle Scholar
  7. Davies, D. B.: 1976, Studia Biophysica, 55, 29Google Scholar
  8. Davies, D. B.: 1978a, Progress in NMR Spectroscopy (Emsley, J. W., Feeney, J. and Sutcliffe, L. H.) Pergamon Press, Oxford, in PressGoogle Scholar
  9. Davies, D. B.: 1978b, Eur. J. Biochem., submitted to PressGoogle Scholar
  10. Davies, D. B.: 1978c, J. Chem. Soc, submitted to PressGoogle Scholar
  11. Davies, D., B.: 1978d, Biochem. Biophys. Acta, submitted to PressGoogle Scholar
  12. Evans, F. E. and Sarma, R. H.: 1976, Nature, 263, 567CrossRefGoogle Scholar
  13. Ezra, F. S., Lee, C-H., Kondo, N. S., Danyluk, S. S. and Sarma, R. H.: 1977, Biochemistry, 16, 1917CrossRefGoogle Scholar
  14. Guéron, M., Chachaty, C. and Tran-Dinh, S.: 1973, Annals. NY Acad. Sci. (USA) 222, 307, and references therein.CrossRefGoogle Scholar
  15. Hruska, F. E.: 1973, Conformations of Biological Molecules and Polymers (Bergmann, E. D. and Pullman, B., eds.) Academic Press, New York, p.345Google Scholar
  16. Hruska, F. E., Wood, D. J. and Singh, H.: 1976, Biochim. Biophys. Acta, 474, 129Google Scholar
  17. Jack, A., Ladner, J. E. and Klug, A.: 1976, J. Mol. Biol., 108, 619CrossRefGoogle Scholar
  18. Klyne, W. and Prelog, V.: 1960, Experentia, 16, 521CrossRefGoogle Scholar
  19. Lee, C-H., Ezra, F. S., Kondo, N. S., Sarma, R. H. and Danyluk, S. S.: 1976, Biochemistry, 15, 3627CrossRefGoogle Scholar
  20. Lemieux, R. U.: 1973, Annals. NY Acad. Sci., 222, 915CrossRefGoogle Scholar
  21. Lüdemann, H-D., Roder, O., Westhof, E., v Goldammer, E., and Müller, A.: 1975, Biophys. Struct. Mechanism, 1, 121CrossRefGoogle Scholar
  22. Schweizer, M. P. and Kreishman, G. P.: 1973, J. Mag. Res., 9, 334Google Scholar
  23. Smith, I. C. P., Mantsch, H. H., Lapper, R. D., Deslauriers, R. and Schleich, T.: 1973, Conformations of Biological Molecules and Polymers (Bergmann, E. D. and Pullman, B., eds.) pp. 381–402, Academic Press, New YorkGoogle Scholar
  24. Sundaralingam, M.: 1973, Conformations of Biological Molecules and Polymers (Bergmann, E. D. and Pullman, B., eds.) pp. 417–455, Academic Press, New York, and references thereinGoogle Scholar
  25. Sundaralingam, M., Pullman, B., Saenger, W., Sasisekharan, V. and Wilson, H. R.: 1973, Conformations of Biological Molecules and Polymers (Bergmann, E. D. and Pullman, B., eds.) pp. 815–820 Academic Press, New YorkGoogle Scholar
  26. Tewari, R., Nanda, R. K. and Govil, G.: 1974, Biopolymers, 13, 2015CrossRefGoogle Scholar
  27. Wood, D. J., Mynott, R. J., Hruska, F. E. and Sarma, R. H.: 1973, Fed. Eur. Biochem. Soc. Letters, 34, 323CrossRefGoogle Scholar
  28. Yathindra, N. and Sundaralingam, M.: 1973, Biopolymers, 12, 297, 2075, 2261CrossRefGoogle Scholar

Copyright information

© D. Reidel Publishing Company, Dordrecht, Holland 1978

Authors and Affiliations

  • David B. Davies
    • 1
  1. 1.Department of ChemistryBirkbeck CollegeLondonUK

Personalised recommendations