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Ascorbic Acid, Glutathione and Lactate in Experimental Ultraviolet Keratitis

  • Martin Reim
  • Eberhard Schuette
  • Gisela Scharsich
  • Marietta Seidl
  • Heinz G. Kesternich
Part of the Documenta Ophthalmologica Proceedings Series book series (DOPS, volume 18)

Abstract

In rabbits, ultraviolet keratitis was produced by irradiation of one eye with an analytical UV lamp. The glutathione (GSH and GSSG) levels of the cornea epithelium did not change before the epithelium showed visible lesions. At that time, about 10 hours after the irradiation, an increase of the GSH and a decrease of the GSSG levels were observed by enzymatic analyses of the corneal epithelium. During the lag period of the UV keratitis, 30 min. and 3 hours after the irradiation, the ascorbic acid (ASC) levels of the stroma and the epithelium of the cornea were decreased. After 4 hours the lactate levels were also slightly decreased. The lactate/pyruvate ratios did not change. The biochemical lesion of UV light could not be identified by the results of this study. But the early decrease of the ASC in the corneal epithelium and stroma may point to a damage of this tissue by perioxides as postulated by several authors.

Keywords

Ascorbic Acid Lactate Level Aqueous Humour Glutathione Level Corneal Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr W. Junk b.v. Publishers 1979

Authors and Affiliations

  • Martin Reim
    • 1
    • 2
  • Eberhard Schuette
    • 1
  • Gisela Scharsich
    • 1
  • Marietta Seidl
    • 1
  • Heinz G. Kesternich
    • 1
  1. 1.AachenGermany
  2. 2.Abteilung für Augenheilkundeder Medizinischen Fakultät der RWTHAachenGermany

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