Ascorbic Acid, Glutathione and Lactate in Experimental Ultraviolet Keratitis
In rabbits, ultraviolet keratitis was produced by irradiation of one eye with an analytical UV lamp. The glutathione (GSH and GSSG) levels of the cornea epithelium did not change before the epithelium showed visible lesions. At that time, about 10 hours after the irradiation, an increase of the GSH and a decrease of the GSSG levels were observed by enzymatic analyses of the corneal epithelium. During the lag period of the UV keratitis, 30 min. and 3 hours after the irradiation, the ascorbic acid (ASC) levels of the stroma and the epithelium of the cornea were decreased. After 4 hours the lactate levels were also slightly decreased. The lactate/pyruvate ratios did not change. The biochemical lesion of UV light could not be identified by the results of this study. But the early decrease of the ASC in the corneal epithelium and stroma may point to a damage of this tissue by perioxides as postulated by several authors.
KeywordsPeroxide Lactate Glutathione Fluorescein Pentobarbital
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- Bachern, A. Ophthalmic Ultraviolet Action Spectra. Am. J. Ophthal. 41: 969–975 (1956).Google Scholar
- Cogan, D.G. & Kinsey, E. Action spectrum of keratitis produced by ultraviolet radiation. Arch. Ophthal., Chicago, 35: 670–677(1946).Google Scholar
- Duke-Elder, W.S. Action on the outer eye. Photoophthalmia. System of Ophthalmology, Vol. IXX. Injuries Part II, Chapter (1972) 918–928.Google Scholar
- Kinsey, V.E. Spectral transmission of the eye to ultraviolet radiations. Arch. Ophthal., Chicago, 39: 508–513 (1948).Google Scholar
- Prie, A. Ascorbic acid content of the cornea. Biochem. J. 40: 96–99 (1946).Google Scholar
- Reim, M., Ashauer, D. The glutathione of the cornea. Arch. Ophthal., Paris, 35: 153–158 (1975).Google Scholar
- Reim, M., Lipp, U. & Venske, G.: Biochemical changes in the cornea after cryotherapy. Proc. XXI. Int. Congr. Ophthal., Mexico 1970; Excerpta Medical Amsterdam 1971, 550–554.Google Scholar