Advertisement

Effect of Varying Medium Potassium on Lens Volume

  • John W. Patterson
Part of the Documenta Ophthalmologica Proceedings Series book series (DOPS, volume 18)

Abstract

Potassium in varying amounts was substituted for sodium in a 305 mOsm saline plus glucose medium. Rat lenses were incubated for 24 hours in the media and the effects on sodium and potassium concentration and on water, sodium and potassium content were determined. Cation potentials and the potential differences across the lens membranes were calculated from the Nernst and Goldman equations. Changes in lens volume are accounted for by changes in the content of potassium with accompanying anion and water. The change in potassium content with increasing potassium in the medium involves two processes — one saturable and identified with the Na, K-pump and one nonsaturable and evident when the pump is saturated. Increases in net potassium content are viewed as being the result of temporary preponderance of potassium influx over efflux that occurs between steady states. A model of volume regulation is described that is consistent with the premises of the double-Donnan model and the data on volume regulation reported for duck red cells.

Keywords

Sodium Content Potassium Content Crystalline Lens Saturable Process Donnan Equilibrium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cotlier, E.B. Kwan & C. Beaty. The lens as an osmometer and the effects of medium osmolarity on water transport, 86Rb efflux and 86Rb transport by the lens. Biochim. Biophys. Acta 150: 705–722 (1968).CrossRefGoogle Scholar
  2. Duncan, G. The site of the ion restricting membranes in the toad lens. Expl. Eye Res. 8: 406–412 (1969).CrossRefGoogle Scholar
  3. Duncan, G. & P.C. Croghan. Mechanisms for the regulation of cell volume with particular reference to the lens. Expl. Eye Res. 8: 421–428 (1969).CrossRefGoogle Scholar
  4. Glynn, I.M. Sodium and potassium movements in human red cells. J. Physiol., Lond. 134: 278–310(1956).PubMedGoogle Scholar
  5. Harris, J.E., L.B. Gehrsitz & L. Nordquist. The in vitro reversal of the lenticular cation shift induced by cold or calcium deficiency. Am. J. Ophthal. 36: 39–50 (1953).PubMedGoogle Scholar
  6. Hightower, K.R. & V.E. Kinsey. Studies on the crystalline lens. XXIII electrogenic potential and cation transport. Expl. Eye Res. 24: 587–593 (1977).CrossRefGoogle Scholar
  7. Hodgkin, A.L. Ionic movements and electrical activity in giant nerve fibres. Proc. R. Soc. B 148: 1–37 (1957).Google Scholar
  8. Hodgkin, A.L. & P. Horowicz. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J. Physiol. Lond. 148: 127–160 (1959).PubMedGoogle Scholar
  9. Kinoshita, J.H., L.O. Merola & S: Hayman. Osmotic effects on amino acid concentrating mechanisms in the rabbit lens. J. biol. Chem. 240: 310–315 (1965).PubMedGoogle Scholar
  10. Kinsey, V.E. Studies on the Crystalline Lens. XIX Quantitative aspects of active and passive transport of sodium Expl. Eye Res. 15: 699–710 (1973).CrossRefGoogle Scholar
  11. Kinsey, V.E. & K.R. Hightower. Studies on the Crystalline lens. XXII characterization of chloride movement based on the pump-leak model. Expl. Eye Res. 23: 425–433 (1976).CrossRefGoogle Scholar
  12. Kinsey, V.E. & K.R. Hightower. Studies on the crystalline lens. XXVI kinetic study showing saturation of the sodium pump. Invest. Ophthal. 17: 186–190 (1978).Google Scholar
  13. Kinsey, V.E. & I.W. McLean. Studies on the crystalline lens. XVI Characterization of active transport and diffusion of potassium, rubidium and cesium. Invest. Ophthal. 9: 769–784 (1970).Google Scholar
  14. Kregenow, F.M. Transport in avain red cells, in membrane transport in red cells. (ed. J.C. Ellory and V.L. Lew) Academic Press, N.Y. 1977.Google Scholar
  15. Macknight, A.D.C. & A. Leaf. Regulation of cellular volume. Physiol. Rev. 57: 510–573 (1977).PubMedGoogle Scholar
  16. Paterson, C.A., M.C. Neville, R.M. Jenkins II & J.P. Cullen. An electrogenic component of potential difference in the rabbit lens. Biochim. Biophys. Acta 375: 309–316 (1975).CrossRefPubMedGoogle Scholar
  17. Patterson, J.W. Effects of amino acid loading on ’Volume Regulation’ in rat lenses. submitted to Expl. Eye Res. (1978).Google Scholar
  18. Patterson, J.W. & D.J. Fournier. The effect of tonicity on lens volume. Invest. Ophthal. 15: 866–869 (1976).PubMedGoogle Scholar
  19. Rae, J.L. The Potential Difference in frog lens. Expl. Eye Res. 15: 485–494 (1973).CrossRefGoogle Scholar
  20. Schenk, D., D.J. Fournier & J.W. Patterson. Tissue culture of rat lenses. Proc. Soc. Exper. Biol. Med. 153: 444–448 (1976).Google Scholar
  21. Shaw, T.I. Potassium movements in washed erythrocytes. J. Physiol., Lond. 129: 464–475 (1955).PubMedGoogle Scholar
  22. Whittam, R. & M.E. Ager. The connection between active cation transport and metabolism in erythrocytes. Biochem. J. 97: 214–227 (1965).PubMedGoogle Scholar

Copyright information

© Dr W. Junk b.v. Publishers 1979

Authors and Affiliations

  • John W. Patterson
    • 1
    • 2
  1. 1.FarmingtonUSA
  2. 2.Department of PhysiologyUniversity of Connecticut Health CenterFarmingtonUSA

Personalised recommendations