Chemical Burn of the Rabbit Cornea Morphometric Studies of the Corneal Stroma
In the rabbit, the corneal burn with a Na OH, 0,5 N solution lead to a diffuse but crowded and durable opacity of this cornea. The ultrastructural observation of this corneal stroma with time shows apparent changes in the fibrillar diameters, alterations of the centered hexagonal fibrillar stacking, with an increase of the interfibrillar distances or sometimes, an agglutination of neighbour fibrils.
But the quantitative study only allows a significant gathering of these facts and reveals that during the first 30 days, the homogeneity is preserved as for the mean fibrillar diameter: 300 ± 85 Å (normal 300 ± b 50 Å).
After the 30th day, the mean value remains approximately the same but with a standard deviation more than 2 times higher, showing an actual heterogeneity: 300 ± 200 Å particularly in the anterior part of the cornea.
The interfibrillar distances inclusively, are generally greater than normally: 625 ± 200 Å, specially during the three first weeks after the burn. However, there exist some zones where the normal fibrillar arrangement is maintened: 540 ± 100 Å, even in the earliest stages. Some lakefree zones are observed.
At last, a small difference between the anterior and posterior parts of the injury is pointed out.
KeywordsHexagonal Fibril Perforation Glutaraldehyde
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